ArticleMolecular biology of the cell1999
Somatic cell mutants resistant to retrovirus replication: intracellular blocks during the early stages of infection.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- FEZ1 Plays Dual Roles in Early HIV-1 Infection by Independently Regulating Capsid Transport and Host Interferon-Stimulated Gene Expression.Journal of virology · 2023Article
- FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression.Cell reports · 2022Article
- The Host RNAs in Retroviral Particles.Viruses · 2016Review
- Mass spectrometry-based proteomic approaches for discovery of HIV-host interactions.Future virology · 2014Article
- Characteristics of the cellular receptor influence the intracellular fate and efficiency of virus infection.Journal of virology · 2013Article
- Article
- TRIM5 and the Regulation of HIV-1 Infectivity.Molecular biology international · 2012Article
- TANK-binding kinase 1 attenuates PTAP-dependent retroviral budding through targeting endosomal sorting complex required for transport-I.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging.PloS one · 2010Article
- The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The Ezrin-radixin-moesin family member ezrin regulates stable microtubule formation and retroviral infection.Journal of virology · 2008Article
- Cell factors stimulate human immunodeficiency virus type 1 reverse transcription in vitro.Journal of virology · 2008Article
- Isolation and characterization of human cells resistant to retrovirus infection.Retrovirology · 2007Article
- Moesin regulates stable microtubule formation and limits retroviral infection in cultured cells.The EMBO journal · 2007Article
- Isolation, characterization, and genetic complementation of a cellular mutant resistant to retroviral infection.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- A phenotypic recessive, post-entry block in rabbit cells that results in aberrant trafficking of HIV-1.Traffic (Copenhagen, Denmark) · 2006Article
- Interaction of moloney murine leukemia virus capsid with Ubc9 and PIASy mediates SUMO-1 addition required early in infection.Journal of virology · 2006Article
- Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane.Molecular biology of the cell · 2005Article
- Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.Journal of virology · 2005Article
- Overexpression of fasciculation and elongation protein zeta-1 (FEZ1) induces a post-entry block to retroviruses in cultured cells.Genes & development · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify cellular functions involved in the early phase of the retroviral life cycle, somatic cell mutants were isolated after selection for resistance to infection. Rat2 fibroblasts were treated with chemical mutagens, and individual virus-resistant clones were recovered after selection for resistance to infection. Two clones were characterized in detail. Both mutant lines were resistant to infection by both ecotropic and amphotropic murine viruses, as well as by human immunodeficiency virus type 1 pseudotypes. One clone showed a strong block to reverse transcription of the retroviral RNA, including formation of the earliest DNA products. The second clone showed normal levels of viral DNA synthesis but did not allow formation of the circular DNAs normally found in the nucleus. Cell fractionation showed that the viral preintegration complex was present in a form that could not be extracted under conditions that readily extracted the complex from wild-type cells. The results suggest that the DNA was trapped in a nonproductive state and excluded from the nucleus of the infected cell. The properties of these two mutant lines suggest that host gene products play important roles both before and after reverse transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.