ArticleOncogene1999
Mutations in serines 15 and 20 of human p53 impair its apoptotic activity.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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Who cites it
72 citing papers in PubMed, 191 citations in OpenAlex.
- Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate.Cell research · 2023Article
- P53 protein and the diseases in central nervous system.Frontiers in genetics · 2022Review
- Dissection of Anti-tumor Activity of Histone Deacetylase Inhibitor SAHA in Nasopharyngeal Carcinoma CellsFrontiers in cell and developmental biology · 2020Article
- Translational Control inInternational journal of molecular sciences · 2019Review
- The p53 mRNA: an integral part of the cellular stress response.Nucleic acids research · 2019Review
- IL-24 Promotes Apoptosis through cAMP-Dependent PKA Pathways in Human Breast Cancer Cells.International journal of molecular sciences · 2018Article
- Dual Role of Nitric Oxide in Regulating the Response of β Cells to DNA Damage.Antioxidants & redox signaling · 2018Review
- Quercetin suppresses DNA double-strand break repair and enhances the radiosensitivity of human ovarian cancer cells via p53-dependent endoplasmic reticulum stress pathway.OncoTargets and therapy · 2018Article
- Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage.Scientific reports · 2017Article
- Article
- A central role of TRAX in the ATM-mediated DNA repair.Oncogene · 2016Article
- Chemical Inhibition of Wild-Type p53-Induced Phosphatase 1 (WIP1/PPM1D) by GSK2830371 Potentiates the Sensitivity to MDM2 Inhibitors in a p53-Dependent Manner.Molecular cancer therapeutics · 2016Article
- Wuho Is a New Member in Maintaining Genome Stability through its Interaction with Flap Endonuclease 1.PLoS biology · 2016Article
- Carbon-ion beams effectively induce growth inhibition and apoptosis in human neural stem cells compared with glioblastoma A172 cells.Journal of radiation research · 2015Article
- Gene expression profile of human lung epithelial cells chronically exposed to single-walled carbon nanotubes.Nanoscale research letters · 2015Article
- Inhibitor of apoptosis-stimulating protein of p53 (iASPP) is required for neuronal survival after axonal injury.PloS one · 2014Article
- Role of p38 and JNK MAPK signaling pathways and tumor suppressor p53 on induction of apoptosis in response to Ad-eIF5A1 in A549 lung cancer cells.Molecular cancer · 2013Article
- ASPP1/2 regulate p53-dependent death of retinal ganglion cells through PUMA and Fas/CD95 activation in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- PRIMA-1 increases cisplatin sensitivity in chemoresistant ovarian cancer cells with p53 mutation: a requirement for Akt down-regulation.Journal of ovarian research · 2013Article
- The critical role of catalase in prooxidant and antioxidant function of p53.Cell death and differentiation · 2013Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation of the p53 tumor suppressor protein is likely to play an important role in regulating its activity. To study the regulatory role of potential phosphorylation sites within the N-terminal transactivation domain of human p53 (hp53), a series of p53 serine mutants were evaluated for transcriptional transactivation and sequence specific DNA binding. The role of these mutations in regulating p53-mediated growth suppression and programmed cell death was examined. This mutational analysis comprised serine residues located at positions 6, 9, 15, 20, 33 and 37 of human p53. Substitution of serine for alanine, either at individual residues or at all six residues together, did not affect the suppression of cell growth and cell transformation, or the ability to bind DNA specifically and to transactivate different promoters, nor did it alter p53 expression. However, the ability of p53 to induce apoptosis was impaired by specific serine substitutions. Mutations in all six N-terminal serines together reduced the apoptotic activity of p53 in H1299 cells by 50%. Analysis of individual mutants revealed that mutations in serine 15 and 20 are primarily responsible for this impairment. Our results suggest that these serines play a role in the regulation of p53-mediated apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.