ArticleThe Journal of physiology1999
Calcium currents during contraction and shortening in enzymatically isolated murine skeletal muscle fibres.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 38 citations in OpenAlex.
- Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.Biomedical optics express · 2016Article
- Localized nuclear and perinuclear Ca(2+) signals in intact mouse skeletal muscle fibers.Frontiers in physiology · 2015Article
- S100A1 deficiency impairs postischemic angiogenesis via compromised proangiogenic endothelial cell function and nitric oxide synthase regulation.Circulation research · 2013Article
- The delayed rectifier potassium conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers.The Journal of general physiology · 2012Article
- Voltage clamp methods for the study of membrane currents and SR Ca(2+) release in adult skeletal muscle fibres.Progress in biophysics and molecular biology · 2012Review
- Functional expression of transgenic 1sDHPR channels in adult mammalian skeletal muscle fibres.The Journal of physiology · 2011Article
- In vivo expression of G-protein beta1gamma2 dimer in adult mouse skeletal muscle alters L-type calcium current and excitation-contraction coupling.The Journal of physiology · 2010Article
- Enhanced muscle shortening and impaired Ca2+ channel function in an acute septic myopathy model.Journal of neurology · 2010Article
- Excitation-contraction coupling in skeletal muscle fibers from adult domestic honeybee.Pflugers Archiv : European journal of physiology · 2009Article
- Rapid Ca2+ flux through the transverse tubular membrane, activated by individual action potentials in mammalian skeletal muscle.The Journal of physiology · 2009Article
- Unloaded speed of shortening in voltage-clamped intact skeletal muscle fibers from wt, mdx, and transgenic minidystrophin mice using a novel high-speed acquisition system.Biophysical journal · 2008Article
- Article
- Ca2+/CaM-dependent inactivation of the skeletal muscle L-type Ca2+ channel (Cav1.1).Pflugers Archiv : European journal of physiology · 2008Article
- NA+- and K+-channels as molecular targets of the alkaloid ajmaline in skeletal muscle fibres.British journal of pharmacology · 2007Article
- A possible role of the junctional face protein JP-45 in modulating Ca2+ release in skeletal muscle.The Journal of physiology · 2006Article
- Voltage-controlled Ca2+ release and entry flux in isolated adult muscle fibres of the mouse.The Journal of physiology · 2005Article
- Mini-dystrophin restores L-type calcium currents in skeletal muscle of transgenic mdx mice.The Journal of physiology · 2004Article
- Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA.Biophysical journal · 2003Article
- Intramembrane charge movement and L-type calcium current in skeletal muscle fibers isolated from control and mdx mice.Biophysical journal · 2003Article
- Numerical analysis of Ca2+ depletion in the transverse tubular system of mammalian muscle.Biophysical journal · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Calcium currents (ICa) were monitored in enzymatically isolated murine toe muscle fibres using the two-microelectrode voltage-clamp technique. ICa was recorded (i) in hypertonic solution to suppress contraction, and (ii) in actively shortening fibres in isotonic solution. 2. In hypertonic solution the threshold potential for ICa was about -30 mV for both 2 and 10 mM external Ca2+ solution. Maximum peak currents measured -12.6 +/- 2.3 nA (mean +/- s.d.; n = 4) in 2 mM Ca2+ and -65 +/- 15 nA (n = 7) in 10 mM Ca2+. The time to peak (TTP) ICa was 96 +/- 22 ms (n = 4) in 2 mM Ca2+ and 132 +/- 13 ms (n = 7) in 10 mM Ca2+. The exponential decay of ICa was similar in 2 and 10 mM Ca2+ with rate constants (tau-1(V)) of 3.7 s-1 (2 mM) and 3.8 s-1 (10 mM) at +10 mV. 3. ICa in isotonic 10 mM Ca2+ solution was recorded by inserting the micropipettes exactly opposite to each other close to the centre of mass of the fibre where negligible contraction-induced movement occurs. 4. In isotonic 10 mM Ca2+ solution ICa had a smaller peak amplitude (-45 +/- 5 nA; n = 7) and faster TTP (82.8 +/- 22.1 ms; n = 7) than in hypertonic solution. The exponential decay of ICa showed a significantly larger tau-1(V) of 6.4 s-1 at +10 mV (P < 0.03). 5. To test for calcium depletion, extracellular Ca2+ was buffered by malic acid in isotonic solution to 9 mM. The decay of ICa had a time constant of 348 +/- 175 ms (n = 14) vs. 107 +/- 24 ms (n = 12; P < 0.001) at 0 mV in unbuffered 10 mM Ca2+ solution. 6. We conclude that calcium depletion from the transverse tubular system contributes significantly to the decay of calcium currents in murine toe muscle fibres under hypertonic as well as isotonic conditions. In the latter, depletion is even more prominent.
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