ArticleThe EMBO journal1999
Post-termination ribosome interactions with the 5'UTR modulate yeast mRNA stability.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 83 citations in OpenAlex.
- Exhaustive identification of conserved upstream open reading frames with potential translational regulatory functions from animal genomes.Scientific reports · 2020Article
- Yeast AP-1 like transcription factors (Yap) and stress response: a current overview.Microbial cell (Graz, Austria) · 2019Review
- Translation initiation events on structured eukaryotic mRNAs generate gene expression noise.Nucleic acids research · 2017Article
- De novo computational identification of stress-related sequence motifs and microRNA target sites in untranslated regions of a plant translatome.Scientific reports · 2017Article
- Harnessing natural sequence variation to dissect posttranscriptional regulatory networks in yeast.G3 (Bethesda, Md.) · 2014Article
- uORFdb--a comprehensive literature database on eukaryotic uORF biology.Nucleic acids research · 2014Article
- Boron-dependent degradation of NIP5;1 mRNA for acclimation to excess boron conditions in Arabidopsis.The Plant cell · 2011Article
- Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes.Nucleic acids research · 2011Article
- Differential regulation and function of 5'-untranslated GR-exon 1 transcripts.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Stress-sensitive regulation of IFRD1 mRNA decay is mediated by an upstream open reading frame.The Journal of biological chemistry · 2010Article
- Properties of untranslated regions of the S. cerevisiae genome.BMC genomics · 2009Article
- Predicting functional upstream open reading frames in Saccharomyces cerevisiae.BMC bioinformatics · 2009Article
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- Modulation of covR expression in Streptococcus mutans UA159.Journal of bacteriology · 2008Article
- Identification of putative regulatory upstream ORFs in the yeast genome using heuristics and evolutionary conservation.BMC bioinformatics · 2007Article
- Evidence for conservation and selection of upstream open reading frames suggests probable encoding of bioactive peptides.BMC genomics · 2006Article
- The uORF-containing thrombopoietin mRNA escapes nonsense-mediated decay (NMD).Nucleic acids research · 2006Article
- The Arabidopsis STV1 protein, responsible for translation reinitiation, is required for auxin-mediated gynoecium patterning.The Plant cell · 2005Article
- Identification and characterization of upstream open reading frames (uORF) in the 5' untranslated regions (UTR) of genes in Saccharomyces cerevisiae.Current genetics · 2005Article
- Hepatitis C virus internal ribosome entry site-dependent translation in Saccharomyces cerevisiae is independent of polypyrimidine tract-binding protein, poly(rC)-binding protein 2, and La protein.Journal of virology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel form of post-transcriptional control is described. The 5' untranslated region (5'UTR) of the Saccharomyces cerevisiae gene encoding the AP1-like transcription factor Yap2 contains two upstream open reading frames (uORF1 and uORF2). The YAP2-type of uORF functions as a cis-acting element that attenuates gene expression at the level of mRNA turnover via termination-dependent decay. Release of post-termination ribosomes from the YAP2 5'UTR causes accelerated decay which is largely independent of the termination modulator gene UPF1. Both of the YAP2 uORFs contribute to the destabilization effect. A G/C-rich stop codon context, which seems to promote ribosome release, allows an uORF to act as a transferable 5'UTR-destabilizing element. Moreover, termination-dependent destabilization is potentiated by stable secondary structure 3' of the uORF stop codon. The potentiation of uORF-mediated destabilization is eliminated if the secondary structure is located further downstream of the uORF, and is also influenced by a modulatory mechanism involving eIF2. Destabilization is therefore linked to the kinetics of acquisition of reinitiation-competence by post-termination ribosomes in the 5'UTR. Our data explain the destabilizing properties of YAP2-type uORFs and also support a more general model for the mode of action of other known uORFs, such as those in the GCN4 mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.