ArticleBiophysical journal1999
Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 178 citations in OpenAlex.
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- Controlling Anomalous Diffusion in Lipid Membranes.Biophysical journal · 2019Article
- Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells.Biomolecules · 2018Article
- Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution.Nature communications · 2016Article
- Fisher information theory for parameter estimation in single molecule microscopy: tutorial.Journal of the Optical Society of America. A, Optics, image science, and vision · 2016Article
- Dynamic heterogeneity and non-Gaussian statistics for acetylcholine receptors on live cell membrane.Nature communications · 2016Article
- Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking.Nucleic acids research · 2015Article
- Actin cytoskeleton reorganization by Syk regulates Fcγ receptor responsiveness by increasing its lateral mobility and clustering.Developmental cell · 2014Article
- Spatial distributions at equilibrium under heterogeneous transient subdiffusion.Frontiers in physiology · 2014Article
- Anomalous versus slowed-down Brownian diffusion in the ligand-binding equilibrium.Biophysical journal · 2013Article
- Crowding effects in vehicular traffic.PloS one · 2012Article
- Correlating anomalous diffusion with lipid bilayer membrane structure using single molecule tracking and atomic force microscopy.The Journal of chemical physics · 2011Article
- Limit of the Accuracy of Parameter Estimation for Moving Single Molecules Imaged by Fluorescence Microscopy.IEEE transactions on signal processing : a publication of the IEEE Signal Processing Society · 2011Article
- HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking.The Journal of biological chemistry · 2010Article
- Spatio-temporal modeling of signaling protein recruitment to EGFR.BMC systems biology · 2010Article
- Elucidating anomalous protein diffusion in living cells with fluorescence correlation spectroscopy-facts and pitfalls.Journal of fluorescence · 2010Article
- Fractional cable equation models for anomalous electrodiffusion in nerve cells: infinite domain solutions.Journal of mathematical biology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-particle tracking (SPT) was used to determine the mobility characteristics of MHC (major histocompatibility complex) class I molecules at the surface of HeLa cells at 22 degrees C and on different time scales. MHC class I was labeled using the Fab fragment of a monoclonal antibody (W6/32), covalently bound to either R-phycoerythrin or fluorescent microspheres, and the particles were tracked using high-sensitivity fluorescence imaging. Analysis of the data for a fixed time interval suggests a reasonable fit to a random diffusion model. The best fit values of the diffusion coefficient D decreased markedly, however, with increasing time interval, demonstrating the existence of anomalous diffusion. Further analysis of the data shows that the diffusion is anomalous over the complete time range investigated, 4-300 s. Fitting the results obtained with the R-phycoerythrin probe to D = D0talpha-1, where Do is a constant and t is the time, gave D0 = (6.7 +/- 4.5) x 10(-11) cm2 s-1 and alpha = 0.49 +/- 0.16. Experiments with fluorescent microspheres were less reproducible and gave slower anomalous diffusion. The R-phycoerythrin probe is considered more reliable for fluorescent SPT because it is small (11 x 8 nm) and monovalent. The type of motion exhibited by the class I molecules will greatly affect their ability to migrate in the plane of the membrane. Anomalous diffusion, in particular, greatly reduces the distance a class I molecule can travel on the time scale of minutes. The present data are discussed in relation to the possible role of diffusion and clustering in T-cell activation.
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