ArticleBiophysical journal1999
Phase imaging by atomic force microscopy: analysis of living homoiothermic vertebrate cells.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 86 citations in OpenAlex.
- Hyperspectral TERS Imaging Reveals Strain Heterogeneity in Individual Nanoplastic Particles.Nano letters · 2025Article
- Applications of microscopy and small angle scattering techniques for the characterisation of supramolecular gels.Beilstein journal of organic chemistry · 2024Review
- Analysis of individual HIV-1 budding event using fast AFM reveals a multiplexed role for VPS4.Biophysical journal · 2022Article
- Review
- Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells.International journal of molecular sciences · 2018Article
- Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.European journal of histochemistry : EJH · 2018Article
- Physicochemical Aspects of the Plasmodium chabaudi-Infected Erythrocyte.BioMed research international · 2015Article
- Atomic force microscopy probing in the measurement of cell mechanics.International journal of nanomedicine · 2010Review
- The avian malaria parasite Plasmodium gallinaceum causes marked structural changes on the surface of its host erythrocyte.Journal of structural biology · 2008Article
- Direct mechanical measurement of geodesic structures in rat mesenchymal stem cells.HFSP journal · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscope-based phase imaging in air is capable of elucidating variations in material properties such as adhesion, friction, and viscoelasticity. However, the interpretation of phase images of specimens in a fluid environment requires clarification. In this report, we systematically analyzed atomic force microscope-derived phase images of mica, glass, and collagen under the same conditions as used for living cells at various tapping forces; the resulting data provide critical information for the interpretation of phase images of living cells. The peripheral regions of COS-1 cells consistently show a more negative phase shift than the glass substrate in phase images at set-point amplitude: free amplitude (Asp/A0) = 0.6-0.8. In addition, at all Asp/A0 values suitable for phase imaging, tapping frequency appears to be high enough to ensure that phase shifts are governed primarily by stiffness. Consequently, phase imaging is capable of high resolution studies of the cellular surface by detecting localized variations in stiffness. We demonstrate that phase imaging of a bifurcating fiber in COS-1 cell cytoplasm is readily capable of a lateral resolution of approximately 30 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.