ArticleBiophysical journal1999
The effects of gramicidin on electroporation of lipid bilayers.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Effects of gramicidin A on the deformation, membrane permeation and membrane stability of lipid vesicles by FeEuropean biophysics journal : EBJ · 2026Article
- The Effect of KcsA Channel on Lipid Bilayer Electroporation Induced by Picosecond Pulse Trains.The Journal of membrane biology · 2020Article
- Investigation of the morphological transition of a phospholipid bilayer membrane in an external electric field via molecular dynamics simulation.Journal of molecular modeling · 2017Article
- Optically transparent polymer devices for in situ assessment of cell electroporation.European biophysics journal : EBJ · 2015Article
- Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 1- Biophysical mechanisms.Biophysical reviews · 2009Review
- Modeling kinetics of subcellular disposition of chemicals.Chemical reviews · 2009Review
- Kinetics, statistics, and energetics of lipid membrane electroporation studied by molecular dynamics simulations.Biophysical journal · 2008Article
- On the effect of prestin on the electrical breakdown of cell membranes.Biophysical journal · 2006Article
- Effect of salicylate on the elasticity, bending stiffness, and strength of SOPC membranes.Biophysical journal · 2005Article
- Membrane electroporation: a molecular dynamics simulation.Biophysical journal · 2005Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of the channel-forming peptide gramicidin D (gD) on the conductance and electroporation thresholds of planar bilayer lipid membranes, made of the synthetic lipid 1-palmitoyl 2-oleoyl phosphatidylcholine (POPC), was studied. High-amplitude ( approximately 200-900 mV) rectangular voltage pulses of 15 ms duration were used to perturb the bilayers and monitor the transmembrane conductance. Electroporation voltage thresholds were found, and conductance was recorded before and after electroporation. Gramicidin was added to the system in peptide/lipid ratios of 1:10, 000, 1:500, and 1:15. The addition of gD in a ratio of 1:10,000 had no effect on electroporation, but ratios of 1:500 and 1:15 significantly increased the thresholds by 16% (p < 0.0001) and 40% (p < 0.0001), respectively. Membrane conductance before electroporation was measurable only after the addition of gD and increased monotonically as the peptide/lipid ratio increased. The effect of gD on the membrane area expansivity modulus (K) was tested using giant unilamellar vesicles (GUVs). When gD was incorporated into the vesicles in a 1:15 ratio, K increased by 110%, consistent with the increase in thresholds predicted by an electromechanical model. These findings suggest that the presence of membrane proteins may affect the electroporation of lipid bilayers by changing their mechanical properties.
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