ArticleBiophysical journal1999
Membrane-pipette interactions underlie delayed voltage activation of mechanosensitive channels in Xenopus oocytes.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Whole-GUV patch-clamping.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Bisphenol A and 4-tert-Octylphenol Inhibit Cx46 Hemichannel Currents.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2015Article
- Anion permeation in calcium-activated chloride channels formed by TMEM16A from Xenopus tropicalis.Pflugers Archiv : European journal of physiology · 2014Article
- Article
- Biophysics and structure of the patch and the gigaseal.Biophysical journal · 2009Article
- Charges dispersed over the permeation pathway determine the charge selectivity and conductance of a Cx32 chimeric hemichannel.The Journal of physiology · 2008Article
- Cooperative gating between single HCN pacemaker channels.The Journal of general physiology · 2006Article
- Two-dimensional kinetic analysis suggests nonsequential gating of mechanosensitive channels in Xenopus oocytes.Biophysical journal · 2001Article
- Stoichiometry of transjunctional voltage-gating polarity reversal by a negative charge substitution in the amino terminus of a connexin32 chimera.The Journal of general physiology · 2000Article
- Calcium-, voltage- and osmotic stress-sensitive currents in Xenopus oocytes and their relationship to single mechanically gated channels.The Journal of physiology · 2000Article
- Voltage-induced membrane displacement in patch pipettes activates mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Membrane stretch affects gating modes of a skeletal muscle sodium channel.Biophysical journal · 1999Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
To investigate the mechanism for the delayed activation by voltage of the predominant mechanosensitive (MS) channel in Xenopus oocytes, currents were recorded from on-cell and excised patches of membrane with the patch clamp technique and from intact oocytes with the two-electrode voltage clamp technique. MS channels could be activated by stretch in inside-out, on-cell, and outside-out patch configurations, using pipettes formed of either borosilicate or soft glass. In inside-out patches formed with borosilicate glass pipettes, depolarizing voltage steps activated MS channels in a cooperative manner after delays of seconds. This voltage-dependent activation was not observed for outside-out patches. Voltage-dependent activation was also not observed when the borosilicate pipettes were either replaced with soft glass pipettes or coated with soft glass. When depolarizing voltage steps were applied to the whole oocyte with a two-electrode voltage clamp, currents that could be attributed to MS channels were not observed. Yet the same depolarizing steps activated MS channels in on-cell patches formed with borosilicate pipettes on the same oocyte. These observations suggest that the delayed cooperative activation of MS channels by depolarization is not an intrinsic property of the channels, but requires interaction between the membrane and patch pipette.
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