ArticleThe Journal of cell biology1999
The unstable F-box protein p58-Ctf13 forms the structural core of the CBF3 kinetochore complex.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 114 citations in OpenAlex.
- Skp1 proteins are structural components of the synaptonemal complex inScience advances · 2024Article
- Oxygen-dependent regulation of E3(SCF)ubiquitin ligases and a Skp1-associated JmjD6 homolog in development of the social amoeba Dictyostelium.The Journal of biological chemistry · 2022Article
- Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction withMolecular and cellular biology · 2020Article
- Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.SLAS technology · 2019Article
- Architecture of the CBF3-centromere complex of the budding yeast kinetochore.Nature structural & molecular biology · 2018Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Structural basis for assembly of the CBF3 kinetochore complex.The EMBO journal · 2018Article
- The crystal structure of the Sgt1-Skp1 complex: the link between Hsp90 and both SCF E3 ubiquitin ligases and kinetochores.Scientific reports · 2017Article
- The Skp1 Homologs SKR-1/2 Are Required for the Caenorhabditis elegans SKN-1 Antioxidant/Detoxification Response Independently of p38 MAPK.PLoS genetics · 2016Article
- Glycosylation of Skp1 promotes formation of Skp1-cullin-1-F-box protein complexes in dictyostelium.Molecular & cellular proteomics : MCP · 2015Article
- Chlorovirus Skp1-binding ankyrin repeat protein interplay and mimicry of cellular ubiquitin ligase machinery.Journal of virology · 2014Article
- Review
- Review
- The ubiquitin-proteasome system of Saccharomyces cerevisiae.Genetics · 2012Review
- Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.The Journal of biological chemistry · 2012Article
- Ndc10 is a platform for inner kinetochore assembly in budding yeast.Nature structural & molecular biology · 2011Article
- Indispensable role for the eukaryotic-like ankyrin domains of the ankyrin B effector of Legionella pneumophila within macrophages and amoebae.Infection and immunity · 2010Article
- Molecular structures and interactions in the yeast kinetochore.Cold Spring Harbor symposia on quantitative biology · 2010Article
- Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa.PLoS pathogens · 2009Article
- Lessons from fungal F-box proteins.Eukaryotic cell · 2009Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Kinetochores are smaller and more accessible experimentally in budding yeast than in any other eukaryote. Believing that simple and complex kinetochores have important structural and functional properties in common, we characterized the structure of CBF3, the essential centromere-binding complex that initiates kinetochore formation in Saccharomyces cerevisiae. We find that the four subunits of CBF3 are multimeric in solution: p23(Skp1) and p58(Ctf13) form a heterodimer, and p64(Cep3) and p110(Ndc10) form homodimers. Subcomplexes involving p58 and each of the other CBF3 subunits can assemble in the absence of centromeric DNA. In these subcomplexes, p58 appears to function as a structural core mediating stable interactions among other CBF3 proteins. p58 has a short half-life in yeast, being subject to ubiquitin-dependent proteolysis, but we find that it is much more stable following association with p64. We propose that p23(Skp1)-p58-p64 complexes constitute the primary pool of active p58 in yeast cells. These complexes can either dissociate, reexposing p58 to the degradation pathway, or can bind to p110 and centromeric DNA, forming a functional CBF3 complex in which p58 is fully protected from degradation. This pathway may constitute an editing mechanism preventing the formation of ectopic kinetochores and ensuring the fidelity of chromosome segregation.
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