ArticleJournal of receptor and signal transduction research1999
Selective alkylation of rat urinary bladder muscarinic receptors with 4-DAMP mustard reveals a contractile function for the M2 muscarinic receptor.
Article in Journal of receptor and signal transduction research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Muscarinic receptor subtypes and signalling involved in the attenuation of isoprenaline-induced rat urinary bladder relaxation.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
- Quantitation of the contractile response mediated by two receptors: M2 and M3 muscarinic receptor-mediated contractions of human gastroesophageal smooth muscle.The Journal of pharmacology and experimental therapeutics · 2009Article
- The use of occupation isoboles for analysis of a response mediated by two receptors: M2 and M3 muscarinic receptor subtype-induced mouse stomach contractions.The Journal of pharmacology and experimental therapeutics · 2008Article
- Regulation of bladder muscarinic receptor subtypes by experimental pathologies.Autonomic & autacoid pharmacology · 2006Article
- Muscarinic receptor transcript and protein density in hypertrophied and atrophied rat urinary bladder.Neurourology and urodynamics · 2006Article
- The M2 muscarinic receptor mediates in vitro bladder contractions from patients with neurogenic bladder dysfunction.American journal of physiology. Regulatory, integrative and comparative physiology · 2004Article
- Hypertrophy changes the muscarinic receptor subtype mediating bladder contraction from M3 toward M2.American journal of physiology. Regulatory, integrative and comparative physiology · 2003Article
- Muscarinic receptors in isolated urinary bladder smooth muscle from different mouse strains.British journal of pharmacology · 2002Article
- Interaction between muscarinic receptor subtype signal transduction pathways mediating bladder contraction.American journal of physiology. Regulatory, integrative and comparative physiology · 2002Article
- The role of M(2)-muscarinic receptors in mediating contraction of the pig urinary bladder in vitro.British journal of pharmacology · 2000Article
- Lower urinary tract physiology and pharmacology.Current urology reports · 2000Review
- The effect of pregnancy and contractile activity on bladder muscarinic receptor subtypes.Neurourology and urodynamics · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Our previous data indicate that M3 muscarinic receptors mediate carbachol induced bladder contractions. The data presented here were obtained by selective alkylation of M3 receptors with 4-DAMP mustard and suggest that the M2 receptor subtype may be involved in inhibition of beta-adrenergic receptor induced relaxation, therefore, allowing recontraction. Alkylation resulted in 85% of M3 receptors and 65% of M2 receptors unable to bind radioligand as demonstrated by subtype selective immunoprecipitation. Rat bladder strips subjected to our alkylation procedure contracted submaximally, and direct carbachol contractions were inhibited by antagonists with affinities consistent with M3 receptor mediated contraction. In contrast, the affinities of antagonists for inhibition of carbachol induced recontractions following isoproterenol stimulated relaxation in the presence of 90 mM KCl, indicated a contractile function for the M2 receptor that was not observed in control strips. In conclusion, these studies demonstrate a possible role for the M2 subtype in bladder smooth muscle contraction.
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