ArticleApplied and environmental microbiology1999
Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Evaluating the effects of synthetic POM cycles and NAD+ kinase expression on fatty alcohol production in Saccharomyces cerevisiae.PloS one · 2025Article
- Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger.Journal of industrial microbiology & biotechnology · 2022Article
- Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production.Synthetic and systems biotechnology · 2022Article
- Intracellular Redox Perturbation inFrontiers in bioengineering and biotechnology · 2020Article
- Article
- High light acclimation of Oryza sativa L. leaves involves specific photosynthetic-sourced changes of NADPH/NADP⁺ in the midvein.Protoplasma · 2015Article
- Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance.Microbial cell factories · 2013Article
- Overexpression of NADH-dependent fumarate reductase improves D-xylose fermentation in recombinant Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2013Article
- In silico profiling of Escherichia coli and Saccharomyces cerevisiae as terpenoid factories.Microbial cell factories · 2013Article
- Characterization of an ntrX mutant of Neisseria gonorrhoeae reveals a response regulator that controls expression of respiratory enzymes in oxidase-positive proteobacteria.Journal of bacteriology · 2013Article
- Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement.Microbial cell factories · 2013Article
- Efficient one-step production of (S)-1-phenyl-1,2-ethanediol from (R)-enantiomer plus NAD(+)-NADPH in-situ regeneration using engineered Escherichia coli.Microbial cell factories · 2012Article
- Progress in metabolic engineering of Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2008Review
- Yeast cell factories for fine chemical and API production.Microbial cell factories · 2008Article
- Role of cultivation media in the development of yeast strains for large scale industrial use.Microbial cell factories · 2005Article
- Improved anaerobic use of arginine by Saccharomyces cerevisiae.Applied and environmental microbiology · 2003Article
- NADH is specifically channeled through the mitochondrial porin channel in Saccharomyces cerevisiae.Journal of bioenergetics and biomembranes · 2002Article
- Cofactor regeneration by a soluble pyridine nucleotide transhydrogenase for biological production of hydromorphone.Applied and environmental microbiology · 2000Article
- Metabolic engineering of Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2000Review
- Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae.Molecular and cellular biochemistryReview
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the physiological effect of the interconversion between the NAD(H) and NADP(H) coenzyme systems in recombinant Saccharomyces cerevisiae expressing the membrane-bound transhydrogenase from Escherichia coli. Our objective was to determine if the membrane-bound transhydrogenase could work in reoxidation of NADH to NAD+ in S. cerevisiae and thereby reduce glycerol formation during anaerobic fermentation. Membranes isolated from the recombinant strains exhibited reduction of 3-acetylpyridine-NAD+ by NADPH and by NADH in the presence of NADP+, which demonstrated that an active enzyme was present. Unlike the situation in E. coli, however, most of the transhydrogenase activity was not present in the yeast plasma membrane; rather, the enzyme appeared to remain localized in the membrane of the endoplasmic reticulum. During anaerobic glucose fermentation we observed an increase in the formation of 2-oxoglutarate, glycerol, and acetic acid in a strain expressing a high level of transhydrogenase, which indicated that increased NADPH consumption and NADH production occurred. The intracellular concentrations of NADH, NAD+, NADPH, and NADP+ were measured in cells expressing transhydrogenase. The reduction of the NADPH pool indicated that the transhydrogenase transferred reducing equivalents from NADPH to NAD+.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.