ArticleProceedings of the National Academy of Sciences of the United States of America1999
GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 99 citations in OpenAlex.
- Engineered hypermutation adapts cyanobacterial photosynthesis to combined high light and high temperature stress.Nature communications · 2023Article
- Light-induced damage to photosystem II at a very low temperature (195 K) depends on singlet oxygen.Physiologia plantarum · 2022Article
- Recent Advances in Understanding the Structural and Functional Evolution of FtsH Proteases.Frontiers in plant science · 2022Review
- Identification and Characterization of a Plastidic Adenine Nucleotide Uniporter (OsBT1-3) Required for Chloroplast Development in the Early Leaf Stage of Rice.Scientific reports · 2017Article
- Identification and Roles of Photosystem II Assembly, Stability, and Repair Factors in Arabidopsis.Frontiers in plant science · 2016Review
- Clues to the functions of plant NDPK isoforms.Naunyn-Schmiedeberg's archives of pharmacology · 2015Review
- Article
- Phylogenetic Analysis of the Thylakoid ATP/ADP Carrier Reveals New Insights into Its Function Restricted to Green Plants.Frontiers in plant science · 2011Article
- Recent advances in understanding the assembly and repair of photosystem II.Annals of botany · 2010Review
- A protein phosphorylation threshold for functional stacking of plant photosynthetic membranes.PloS one · 2010Article
- Role of thylakoid ATP/ADP carrier in photoinhibition and photoprotection of photosystem II in Arabidopsis.Plant physiology · 2010Article
- Solute transporters in plant thylakoid membranes: Key players during photosynthesis and light stress.Communicative & integrative biology · 2010Article
- Phosphorylation of photosystem II controls functional macroscopic folding of photosynthetic membranes in Arabidopsis.The Plant cell · 2009Article
- Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp. ORS 278.Photosynthesis research · 2009Article
- Consequences of C4 differentiation for chloroplast membrane proteomes in maize mesophyll and bundle sheath cells.Molecular & cellular proteomics : MCP · 2008Article
- Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii.Plant physiology · 2008Article
- The exposed N-terminal tail of the D1 subunit is required for rapid D1 degradation during photosystem II repair in Synechocystis sp PCC 6803.The Plant cell · 2007Article
- The extrinsic proteins of Photosystem II.Photosynthesis research · 2007Review
- Formation of DEG5 and DEG8 complexes and their involvement in the degradation of photodamaged photosystem II reaction center D1 protein in Arabidopsis.The Plant cell · 2007Article
- Temporal gene expression profiling of the wheat leaf rust pathosystem using cDNA microarray reveals differences in compatible and incompatible defence pathways.International journal of plant genomics · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Even though light is the driving force in photosynthesis, it also can be harmful to plants. The water-splitting photosystem II is the main target for this light stress, leading to inactivation of photosynthetic electron transport and photooxidative damage to its reaction center. The plant survives through an intricate repair mechanism involving proteolytic degradation and replacement of the photodamaged reaction center D1 protein. Based on experiments with isolated chloroplast thylakoid membranes and photosystem II core complexes, we report several aspects concerning the rapid turnover of the D1 protein. (i) The primary cleavage step is a GTP-dependent process, leading to accumulation of a 23-kDa N-terminal fragment. (ii) Proteolysis of the D1 protein is inhibited below basal levels by nonhydrolyzable GTP analogues and apyrase treatment, indicating the existence of endogenous GTP tightly bound to the thylakoid membrane. This possibility was corroborated by binding studies. (iii) The proteolysis of the 23-kDa primary degradation fragment (but not of the D1 protein) is an ATP- and zinc-dependent process. (iv) D1 protein degradation is a multienzyme event involving a strategic (primary) protease and a cleaning-up (secondary) protease. (v) The chloroplast FtsH protease is likely to be involved in the secondary degradation steps. Apart from its significance for understanding the repair of photoinhibition, the discovery of tightly bound GTP should have general implications for other regulatory reactions and signal transduction pathways associated with the photosynthetic membrane.
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