ArticleProceedings of the National Academy of Sciences of the United States of America1999
Toward functional genomics in bacteria: analysis of gene expression in Escherichia coli from a bacterial artificial chromosome library of Bacillus cereus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 124 citations in OpenAlex.
- Proposal of metagenomic-origin LRA-5 as a precursor of active β-lactamases through Tyr69Gln and Val166Glu amino acid substitutions: a functional and structural analysis.Antimicrobial agents and chemotherapy · 2026Article
- Modular Assembly of Synthetic Secondary Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity.Methods and protocols · 2019Article
- Identification and characterization of a novel alkalistable and salt-tolerant esterase from the deep-sea hydrothermal vent of the East Pacific Rise.MicrobiologyOpen · 2018Article
- Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library.Biotechnology reports (Amsterdam, Netherlands) · 2017Article
- Crystal structure and kinetic analysis of the class B3 di-zinc metallo-β-lactamase LRA-12 from an Alaskan soil metagenome.PloS one · 2017Article
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- A function-based screen for seeking RubisCO active clones from metagenomes: novel enzymes influencing RubisCO activity.The ISME journal · 2015Article
- Host-microbe interactions in distal airways: relevance to chronic airway diseases.European respiratory review : an official journal of the European Respiratory Society · 2015Review
- Application of metagenomics in the human gut microbiome.World journal of gastroenterology · 2015Review
- Strategies and approaches in plasmidome studies-uncovering plasmid diversity disregarding of linear elements?Frontiers in microbiology · 2015Review
- Insights into novel antimicrobial compounds and antibiotic resistance genes from soil metagenomes.Frontiers in microbiology · 2014Review
- Gut-liver axis: role of inflammasomes.Journal of clinical and experimental hepatology · 2013Review
- Article
- The use of metagenomic approaches to analyze changes in microbial communities.Microbiology insights · 2013Review
- Variable within- and between-herd diversity of CTX-M cephalosporinase-bearing Escherichia coli isolates from dairy cattle.Applied and environmental microbiology · 2012Article
- Bacterial artificial chromosome libraries of pulse crops: characteristics and applications.Journal of biomedicine & biotechnology · 2012Review
- Marine genomics: at the interface of marine microbial ecology and biodiscovery.Microbial biotechnology · 2010Review
- Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.Applied and environmental microbiology · 2008Article
- Molecular epidemiology of blaCMY-2 plasmids carried by Salmonella enterica and Escherichia coli isolates from cattle in the Pacific Northwest.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
As the study of microbes moves into the era of functional genomics, there is an increasing need for molecular tools for analysis of a wide diversity of microorganisms. Currently, biological study of many prokaryotes of agricultural, medical, and fundamental scientific interest is limited by the lack of adequate genetic tools. We report the application of the bacterial artificial chromosome (BAC) vector to prokaryotic biology as a powerful approach to address this need. We constructed a BAC library in Escherichia coli from genomic DNA of the Gram-positive bacterium Bacillus cereus. This library provides 5.75-fold coverage of the B. cereus genome, with an average insert size of 98 kb. To determine the extent of heterologous expression of B. cereus genes in the library, we screened it for expression of several B. cereus activities in the E. coli host. Clones expressing 6 of 10 activities tested were identified in the library, namely, ampicillin resistance, zwittermicin A resistance, esculin hydrolysis, hemolysis, orange pigment production, and lecithinase activity. We analyzed selected BAC clones genetically to identify rapidly specific B. cereus loci. These results suggest that BAC libraries will provide a powerful approach for studying gene expression from diverse prokaryotes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.