ArticleProceedings of the National Academy of Sciences of the United States of America1999
Multiplex detection of four pathogenic retroviruses using molecular beacons.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
84 citing papers in PubMed.
- Design Principles of Nanosensors for Multiplex Detection of Contaminants in Food.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- A bibliometric analysis of nucleic acid probe and its applications in oncology: towards more precise molecular medicine.Discover oncology · 2025Review
- Turn-on RNA Mango Beacons forRNA (New York, N.Y.) · 2024Article
- Simultaneous Detection of Influenza A/B, Respiratory Syncytial Virus, and SARS-CoV-2 in Nasopharyngeal Swabs by One-Tube Multiplex Reverse Transcription Polymerase Chain Reaction.Tropical medicine and infectious disease · 2023Article
- Highly multiplex PCR assays by coupling the 5'-flap endonuclease activity ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Novel nucleic acid origami structures and conventional molecular beacon-based platforms: a comparison in biosensing applications.Analytical and bioanalytical chemistry · 2021Review
- Article
- Coronavirus Disease 2019 (COVID-19) Diagnostic Tools: A Focus on Detection Technologies and Limitations.Current issues in molecular biology · 2021Review
- Article
- Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids.Frontiers in microbiology · 2018Review
- Single Nucleotide Polymorphism Genotyping in Single-Molecule Electronic Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Field-deployable, quantitative, rapid identification of active Ebola virus infection in unprocessed blood.Chemical science · 2017Article
- Establishment of an accurate and fast detection method using molecular beacons in loop-mediated isothermal amplification assay.Scientific reports · 2017Article
- MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA.Theranostics · 2017Article
- Multiplex Reverse Transcriptase-PCR for Simultaneous Detection of Hepatitis B, C, and E Virus.Journal of clinical and experimental hepatology · 2016Article
- Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus.Journal of food science and technology · 2015Article
- DNA strands with alternating incorporations of LNA and 2'-Chemical science · 2013Article
- Simultaneous detection of major enteric viruses using a combimatrix microarray.Journal of microbiology (Seoul, Korea) · 2012Article
- A fluorescent reporter of ATP binding-competent receptor kinases.Bioorganic & medicinal chemistry letters · 2012Article
- Design and Development of a Multiplex Real-Time PCR Assay for Detection of HIV-1 and HCV Using Molecular Beacons.Indian journal of microbiology · 2012Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We describe a multiplex nucleic acid assay that identifies and determines the abundance of four different pathogenic retroviruses (HIV-1, HIV-2, and human T-lymphotrophic virus types I and II). Retroviral DNA sequences are amplified in a single, sealed tube by simultaneous PCR assays, and the resulting amplicons are detected in real time by the hybridization of four differently colored, amplicon-specific molecular beacons. The color of the fluorescence generated in the course of amplification identifies which retroviruses are present, and the number of thermal cycles required for the intensity of each color to rise significantly above background provides an accurate measure of the number of copies of each retroviral sequence that were present originally in the sample. Fewer than 10 retroviral genomes can be detected. Moreover, 10 copies of a rare retrovirus can be detected in the presence of 100, 000 copies of an abundant retrovirus. Ninety-six samples can be analyzed in 3 hr on a single plate, and the use of a closed-tube format eliminates crossover contamination. Utilizing previously well characterized clinical samples, we demonstrate that each of the pathogenic retroviruses can be identified correctly and no false positives occur. This assay enables the rapid and reliable screening of donated blood and transplantable tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.