ArticleProceedings of the National Academy of Sciences of the United States of America1999
The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 89 citations in OpenAlex.
- CD127International journal of molecular sciences · 2026Article
- Control of Viral Infection by Natural Killer Cell Inhibitory Receptors.Cell reports · 2020Article
- Article
- Immunoreceptor tyrosine-based inhibitory motif-dependent functions of an MHC class I-specific NK cell receptor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Genetic dissection of NK cell responses.Frontiers in immunology · 2012Article
- Identification of the nephropathy-susceptibility locus HIVAN4.Journal of the American Society of Nephrology : JASN · 2011Article
- Altered NK cell development and enhanced NK cell-mediated resistance to mouse cytomegalovirus in NKG2D-deficient mice.Immunity · 2009Article
- Ly49h-deficient C57BL/6 mice: a new mouse cytomegalovirus-susceptible model remains resistant to unrelated pathogens controlled by the NK gene complex.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Continuous engagement of a self-specific activation receptor induces NK cell tolerance.The Journal of experimental medicine · 2008Article
- NKR-P1 biology: from prototype to missing self.Immunologic research · 2006Review
- The natural killer complex regulates severe malarial pathogenesis and influences acquired immune responses to Plasmodium berghei ANKA.Infection and immunity · 2005Article
- Direct sequence comparison of two divergent class I MHC natural killer cell receptor haplotypes.Genes and immunity · 2005Article
- Natural killer cell immune responses.Immunologic research · 2005Review
- Genomic organization and evolutionary analysis of Ly49 genes encoding the rodent natural killer cell receptors: rapid evolution by repeated gene duplication.Immunogenetics · 2004Article
- Immune functions encoded by the natural killer gene complex.Nature reviews. Immunology · 2003Review
- Genetic control of innate immune responses against cytomegalovirus: MCMV meets its match.Genes and immunity · 2002Review
- Recognition of a virus-encoded ligand by a natural killer cell activation receptor.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Activating Ly-49D and inhibitory Ly-49A natural killer cell receptors demonstrate distinct requirements for interaction with H2-D(d).The Journal of experimental medicine · 2000Article
- Nonstochastic coexpression of activation receptors on murine natural killer cells.The Journal of experimental medicine · 2000Article
- Ly49 gene expression in different inbred mouse strains.Immunologic research · 2000Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Previously, we established that natural killer (NK) cells from C57BL/6 (B6), but not BALB/c, mice lysed Chinese hamster ovary (CHO) cells, and we mapped the locus that determines this differential CHO-killing capacity to the NK gene complex on chromosome 6. The localization of Chok in the NK gene complex suggested that it may encode either an activating or an inhibitory receptor. Here, results from a lectin-facilitated lysis assay predicted that Chok is an activating B6 NK receptor. Therefore, we immunized BALB/c mice with NK cells from BALB.B6-Cmv1(r) congenic mice and generated a mAb, designated 4E4, that blocked B6-mediated CHO lysis. mAb 4E4 also redirected lysis of Daudi targets, indicating its reactivity with an activating NK cell receptor. Furthermore, only the 4E4(+) B6 NK cell subset mediated CHO killing, and this lysis was abrogated by preincubation with mAb 4E4. Flow cytometric analysis indicated that mAb 4E4 specifically reacts with Ly-49D but not Ly-49A, B, C, E, G, H, or I transfectants. Finally, gene transfer of Ly-49DB6 into BALB/c NK cells conferred cytotoxic capacity against CHO cells, thus establishing that the Ly-49D receptor is sufficient to activate NK cells to lyse this target. Hence, Ly-49D is the Chok gene product and is a mouse NK cell receptor capable of directly triggering natural killing.
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