ArticleProceedings of the National Academy of Sciences of the United States of America1999
Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
117 citing papers in PubMed, 320 citations in OpenAlex.
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- PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.Nature communications · 2025Article
- Photoswitchable intein for light control of covalent protein binding and cleavage.Nature communications · 2025Article
- Real-Time and Site-Specific Perturbation of Dynamic Subcellular Compartments Using Femtosecond Pulses.Small science · 2025Article
- Loss of Heterozygosity associated with ubiquitous environments in yeast.PLoS genetics · 2025Article
- Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells.Cancer medicine · 2025Article
- Effects and Mechanisms of Imperatorin on Vitrified Mouse Oocytes.Animals : an open access journal from MDPI · 2025Article
- Damage processes in extended laser exposures using anFrontiers in ophthalmology · 2025Article
- Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine.International journal of molecular sciences · 2024Article
- Light Pollution and Oxidative Stress: Effects on Retina and Human Health.Antioxidants (Basel, Switzerland) · 2024Review
- Harnessing artificial intelligence to reduce phototoxicity in live imaging.Journal of cell science · 2024Article
- Potential consequences of phototoxicity on cell function during live imaging of intestinal organoids.PloS one · 2024Article
- Unified modeling of photothermal and photochemical damage.Frontiers in ophthalmology · 2024Article
- Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2024Article
- Spatiotemporal distribution of reactive oxygen species production, delivery, and use in Arabidopsis root hairs.Plant physiology · 2023Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Violet-blue light is toxic to mammalian cells, and this toxicity has been linked with cellular production of H2O2. In this report, we show that violet-blue light, as well as UVA, stimulated H2O2 production in cultured mouse, monkey, and human cells. We found that H2O2 originated in peroxisomes and mitochondria, and it was enhanced in cells overexpressing flavin-containing oxidases. These results support the hypothesis that photoreduction of flavoproteins underlies light-induced production of H2O2 in cells. Because H2O2 and its metabolite, hydroxyl radicals, can cause cellular damage, these reactive oxygen species may contribute to pathologies associated with exposure to UVA, violet, and blue light. They may also contribute to phototoxicity often encountered during light microscopy. Because multiphoton excitation imaging with 1,047-nm wavelength prevented light-induced H2O2 production in cells, possibly by minimizing photoreduction of flavoproteins, this technique may be useful for decreasing phototoxicity during fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.