ArticleProceedings of the National Academy of Sciences of the United States of America1999
Making artificial antibodies: a format for phage display of combinatorial heterodimeric arrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed.
- Evolution and synthetic biology.Current opinion in microbiology · 2023Review
- Cryo-EM structure of a bacteriophage M13 mini variant.Nature communications · 2023Article
- Review
- A New Vaccination Method Based on Phage NgoΦ6 and Its Phagemid Derivatives.Frontiers in microbiology · 2022Article
- Protein Engineering: Advances in Phage Display for Basic Science and Medical Research.Biochemistry. Biokhimiia · 2022Review
- Exhaustive Search of the Receptor Ligands by the CyCLOPS (Cytometry Cell-Labeling Operable Phage Screening) Technique.International journal of molecular sciences · 2020Article
- Plant/Bacterial Virus-Based Drug Discovery, Drug Delivery, and Therapeutics.Pharmaceutics · 2019Review
- Generating FN3-Based Affinity Reagents Through Phage Display.Current protocols in chemical biology · 2018Article
- Multivalent pIX phage display selects for distinct and improved antibody properties.Scientific reports · 2016Article
- Design of virus-based nanomaterials for medicine, biotechnology, and energy.Chemical Society reviews · 2016Review
- Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.Frontiers in microbiology · 2015Review
- Immobilization techniques for microarray: challenges and applications.Sensors (Basel, Switzerland) · 2014Review
- Synthetic phage for tissue regeneration.Mediators of inflammation · 2014Review
- Oligopeptide m13 phage display in pathogen research.Viruses · 2013Review
- Simultaneous display of two large proteins on the head and tail of bacteriophage lambda.BMC biotechnology · 2013Article
- Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly.Nanotechnology · 2012Article
- DeltaPhage--a novel helper phage for high-valence pIX phagemid display.Nucleic acids research · 2012Article
- M13 bacteriophage display framework that allows sortase-mediated modification of surface-accessible phage proteins.Bioconjugate chemistry · 2012Article
- Detection of biomarkers using recombinant antibodies coupled to nanostructured platforms.Nano reviews · 2012Article
- Site directed biotinylation of filamentous phage structural proteins.Virology journal · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The gene VII protein (pVII) and gene IX protein (pIX) are associated closely on the surface of filamentous bacteriophage that is opposite of the end harboring the widely exploited pIII protein. We developed a phagemid format wherein antibody heavy- and light-chain variable regions were fused to the amino termini of pVII and pIX, respectively. Significantly, the fusion proteins interacted to form a functional Fv-binding domain on the phage surface. Our approach will be applicable to the display of generic peptide and protein libraries that can form combinatorial heterodimeric arrays. Consequently, it represents a first step toward artificial antibodies and the selection of novel biological activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.