ArticleInfection and immunity1999
Chitinase secretion by encysting Entamoeba invadens and transfected Entamoeba histolytica trophozoites: localization of secretory vesicles, endoplasmic reticulum, and Golgi apparatus.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 99 citations in OpenAlex.
- Novel Insights into the Wattle and Daub Model ofPathogens (Basel, Switzerland) · 2023Article
- Anaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol.PLoS pathogens · 2021Article
- Article
- Unique Endomembrane Systems and Virulence in Pathogenic Protozoa.Life (Basel, Switzerland) · 2021Review
- EhRab21 associates with the Golgi apparatus in Entamoeba histolytica.Parasitology research · 2020Article
- Lipids inFrontiers in cellular and infection microbiology · 2020Review
- Phosphorylation of eukaryotic initiation factor-2α in response to endoplasmic reticulum and nitrosative stress in the human protozoan parasite, Entamoeba histolytica.Molecular and biochemical parasitology · 2019Article
- A putative calcium-ATPase of the secretory pathway family may regulate calcium/manganese levels in the Golgi apparatus of Entamoeba histolytica.Parasitology research · 2018Article
- A sophisticated, differentiated Golgi in the ancestor of eukaryotes.BMC biology · 2018Article
- Flow cytometric characterization of encystation in Entamoeba invadens.Molecular and biochemical parasitology · 2017Article
- Membrane Trafficking Modulation during Entamoeba Encystation.Scientific reports · 2017Article
- The cell surface proteome of Entamoeba histolytica.Molecular & cellular proteomics : MCP · 2014Article
- Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors.Journal of tropical medicine · 2013Article
- Silencing of Entamoeba histolytica glucosamine 6-phosphate isomerase by RNA interference inhibits the formation of cyst-like structures.BioMed research international · 2013Article
- Transcriptome analysis of encystation in Entamoeba invadens.PloS one · 2013Article
- Actin, RhoA, and Rab11 participation during encystment in Entamoeba invadens.BioMed research international · 2013Article
- Entamoeba histolytica cell surface calreticulin binds human c1q and functions in amebic phagocytosis of host cells.Infection and immunity · 2012Article
- Evolution and diversity of the Golgi.Cold Spring Harbor perspectives in biology · 2011Article
- Different structure and mRNA expression of Entamoeba invadens chitinases in the encystation and excystation.Parasitology research · 2011Article
- Entamoeba histolytica calreticulin: an endoplasmic reticulum protein expressed by trophozoites into experimentally induced amoebic liver abscesses.Parasitology research · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Entamoeba histolytica, the protozoan parasite that phagocytoses bacteria and host cells, has a vesicle/vacuole-filled cytosol like that of macrophages. In contrast, the infectious cyst form has four nuclei and a chitin wall. Here, anti-chitinase antibodies identified hundreds of small secretory vesicles in encysting E. invadens parasites and in E. histolytica trophozoites overexpressing chitinase under an actin gene promoter. Abundant small secretory vesicles were also identified with antibodies to the surface antigen Ariel and with a fluorescent substrate of cysteine proteinases. Removal of an N-terminal signal sequence directed chitinase to the cytosol. Addition of a C-terminal KDEL peptide, identified on amebic BiP, retained chitinase in a putative endoplasmic reticulum, which was composed of a few vesicles of mixed sizes. A putative Golgi apparatus, which was Brefeldin A sensitive and composed of a few large, perinuclear vesicles, was identified with antibodies to ADP-ribosylating factor and to epsilon-COP. We conclude that the amebic secretory pathway is similar to those of other eukaryotic cells, even if its appearance is somewhat different.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.