ArticleInfection and immunity1999
Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 102 citations in OpenAlex.
- Comparative Effects ofInternational journal of molecular sciences · 2026Article
- Propofol-induced osteogenic differentiation in dental pulp stem cells: Modulation of MAPK signaling under inflammatory conditions.Journal of dental sciences · 2025Article
- Locally Delivered Metabolite Derivative Promotes Bone Regeneration in Aged Mice.ACS applied bio materials · 2022Article
- Toll-like Receptor 4, Osteoblasts and Leukemogenesis; the Lesson from Acute Myeloid Leukemia.Molecules (Basel, Switzerland) · 2022Review
- Xanthine Derivative KMUP-1 Attenuates Experimental Periodontitis by Reducing Osteoclast Differentiation and Inflammation.Frontiers in pharmacology · 2022Article
- Regulation of Bone Cell Differentiation and Activation by Microbe-Associated Molecular Patterns.International journal of molecular sciences · 2021Review
- lncRNA NEAT1 ameliorates LPS‑induced inflammation in MG63 cells by activating autophagy and suppressing the NLRP3 inflammasome.International journal of molecular medicine · 2021Article
- Periodontal Injection of Lipopolysaccharide Promotes Arthritis Development in Mice.Inflammation · 2019Article
- KR-12-a5 Reverses Adverse Effects of Lipopolysaccharides on HBMSC Osteogenic Differentiation by Influencing BMP/Smad and P38 MAPK Signaling Pathways.Frontiers in pharmacology · 2019Article
- A biological study establishing the endotoxin limit for osteoblast and adipocyte differentiation of human mesenchymal stem cells.Regenerative therapy · 2018Article
- Resveratrol Attenuates Lipopolysaccharides (LPS)-Induced Inhibition of Osteoblast Differentiation in MC3T3-E1 Cells.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Role of Toll-Like Receptor 4 on Osteoblast Metabolism and Function.Frontiers in physiology · 2018Review
- A biological study establishing the endotoxin limit for in vitro proliferation of human mesenchymal stem cells.Regenerative therapy · 2017Article
- Porphyromonas gingivalis lipopolysaccharide regulates ephrin/Eph signalling in human periodontal ligament fibroblasts.Journal of periodontal research · 2017Article
- Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts followingInternational journal of nanomedicine · 2017Article
- Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.Jundishapur journal of microbiology · 2015Article
- Gene expression patterns in bone following lipopolysaccharide stimulation.Cellular & molecular biology letters · 2014Article
- Porphyromonas gingivalis infection increases osteoclastic bone resorption and osteoblastic bone formation in a periodontitis mouse model.BMC oral health · 2014Article
- Lipopolysaccharide (LPS) induces the apoptosis and inhibits osteoblast differentiation through JNK pathway in MC3T3-E1 cells.Inflammation · 2014Article
- TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions.Bone research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipopolysaccharide from Porphyromonas gingivalis (P-LPS), an important pathogenic bacterium, is closely associated with inflammatory destruction of periodontal tissues. P-LPS induces the release of cytokines and local factors from inflammatory cells, stimulates osteoclastic-cell differentiation, and causes alveolar bone resorption. However, the effect of P-LPS on osteoblastic-cell differentiation remains unclear. In this study, we investigated the effect of P-LPS extract prepared by the hot-phenol-water method, on the differentiation of primary fetal rat calvaria (RC) cells, which contain a subpopulation of osteoprogenitor cells, into osteoblastic cells. P-LPS extract significantly inhibited bone nodule (BN) formation and the activity of alkaline phosphatase (ALPase), an osteoblastic marker, in a dose-dependent manner (0 to 100 ng of P-LPS extract per ml). P-LPS extract (100 ng/ml) significantly decreased BN formation to 27% of the control value and inhibited ALPase activity to approximately 60% of the control level on days 10 to 21 but did not affect RC cell proliferation and viability. P-LPS extract time-dependently suppressed the expression of ALPase mRNA, with an inhibitory pattern similar to that of enzyme activity. The expression of mRNAs for osteocalcin and osteopontin, matrix proteins related to bone metabolism, was markedly suppressed by P-LPS extract. Furthermore, P-LPS extract increased the expression of mRNAs for CD14, LPS receptor, and interleukin-1beta in RC cells. These results indicate that P-LPS inhibits osteoblastic-cell differentiation and suggest that LPS-induced bone resorption in periodontal disease may be mediated by effects on osteoblastic as well as osteoclastic cells.
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