ArticleClinical and experimental immunology1999
Use of a whole blood assay to evaluate in vitro T cell responses to new leprosy skin test antigens in leprosy patients and healthy subjects.
Article in Clinical and experimental immunology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Transcription factors STAT-4, STAT-6 and CREB regulate Th1/Th2 response in leprosy patients: effect of M. leprae antigens.BMC infectious diseases · 2019Article
- Whole blood assay and visceral leishmaniasis: Challenges and promises.Immunobiology · 2014Review
- The challenge of producing skin test antigens with minimal resources suitable for human application against a neglected tropical disease; leprosy.PLoS neglected tropical diseases · 2014Article
- Gene expression profile and immunological evaluation of unique hypothetical unknown proteins of Mycobacterium leprae by using quantitative real-time PCR.Clinical and vaccine immunology : CVI · 2013Article
- PARK2 mediates interleukin 6 and monocyte chemoattractant protein 1 production by human macrophages.PLoS neglected tropical diseases · 2013Article
- Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting.BMC infectious diseases · 2012Article
- Early deaths during tuberculosis treatment are associated with depressed innate responses, bacterial infection, and tuberculosis progression.The Journal of infectious diseases · 2011Article
- Article
- Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.PloS one · 2009Article
- Antigen-specific T-cell responses of leprosy patients.Clinical and vaccine immunology : CVI · 2008Article
- Extended culture enhances sensitivity of a gamma interferon assay for latent Mycobacterium tuberculosis infection.Clinical and vaccine immunology : CVI · 2007Article
- Immune markers measured before treatment predict outcome of intensive phase tuberculosis therapy.Clinical and experimental immunology · 2006Article
- Antigen discovery: a postgenomic approach to leprosy diagnosis.Infection and immunity · 2006Article
- A postgenomic approach to identification of Mycobacterium leprae-specific peptides as T-cell reagents.Infection and immunity · 2000Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Development of an immunological tool to detect infection with Mycobacterium leprae would greatly benefit leprosy control programmes, as demonstrated by the contribution of the tuberculin test to tuberculosis control. In a new approach to develop a 'tuberculin-like' reagent for use in leprosy, two new fractions of M. leprae depleted of cross-reactive and immunomodulatory lipids- MLSA-LAM (cytosol-derived) and MLCwA (cell wall-derived)-have been produced in a form suitable for use as skin test reagents. T cell responses (interferon-gamma (IFN-gamma) and lymphoproliferation) to these two new fractions were evaluated in a leprosy-endemic area of Nepal using a simple in vitro whole blood test. The two fractions were shown to be highly potent T cell antigens in subjects exposed to M. leprae-paucibacillary leprosy patients and household contacts. Responses to the fractions decreased towards the lepromatous pole of leprosy. Endemic control subjects also showed high responses to the fractions, indicating high exposure to M. leprae, or cross-reactive mycobacterial antigens, in this Nepali population. The new fractions, depleted of lipids and lipoarabinomannan (LAM) gave enhanced responses compared with a standard M. leprae sonicate. The cell wall fraction appeared a more potent antigen than the cytosol fraction, which may be due to the predominance of the 65-kD GroEL antigen in the cell wall. The whole blood assay proved a robust field tool and a useful way of evaluating such reagents prior to clinical trials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.