ArticleRNA (New York, N.Y.)1999
The 5' end of the 18S rRNA can be positioned from within the mature rRNA.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Functional regions in the 5' external transcribed spacer of yeast pre-rRNA.RNA (New York, N.Y.) · 2020Article
- The human RNA helicase DHX37 is required for release of the U3 snoRNP from pre-ribosomal particles.RNA biology · 2019Article
- Maturation of the 90S pre-ribosome requires Mrd1 dependent U3 snoRNA and 35S pre-rRNA structural rearrangements.Nucleic acids research · 2018Article
- hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0.RNA biology · 2015Article
- The initial U3 snoRNA:pre-rRNA base pairing interaction required for pre-18S rRNA folding revealed by in vivo chemical probing.Nucleic acids research · 2011Article
- An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA.Nucleic acids research · 2005Article
- Role of pre-rRNA base pairing and 80S complex formation in subnucleolar localization of the U3 snoRNP.Molecular and cellular biology · 2004Article
- Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing.Molecular and cellular biology · 2001Article
- Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2.RNA (New York, N.Y.) · 2000Article
- Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.Molecular and cellular biology · 1999Article
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
In yeast, the 5' end of the mature 18S rRNA is generated by endonucleolytic cleavage at site A1, the position of which is specified by two distinct signals. An evolutionarily conserved sequence immediately upstream of the cleavage site has previously been shown to constitute one of these signals. We report here that a conserved stem-loop structure within the 5' region of the 18S rRNA is recognized as a second positioning signal. Mutations predicted to either extend or destabilize the stem inhibited the normal positioning of site A1 from within the 18S rRNA sequence, as did substitution of the loop nucleotides. In addition, these mutations destabilized the mature 18S rRNA, indicating that recognition of the stem-loop structure is also required for 18S rRNA stability. Several mutations tested reduced the efficiency of pre-rRNA cleavage at site A1. There was, however, a poor correlation between the effects of the different mutations on the efficiency of cleavage and on the choice of cleavage site, indicating that these involve recognition of the stem-loop region by distinct factors. In contrast, the cleavages at sites A1 and A2 are coupled and the positioning signals appear to be similar, suggesting that both cleavages may be carried out by the same endonuclease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.