ArticleRNA (New York, N.Y.)1999
Crystal structure of acceptor stem of tRNA(Ala) from Escherichia coli shows unique G.U wobble base pair at 1.16 A resolution.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- The XNA alphabet.Nucleic acids research · 2025Review
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- Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Construction of anti-codon table of the plant kingdom and evolution of tRNA selenocysteine (tRNABMC genomics · 2020Article
- Nucleobase carbonyl groups are poor MgRNA (New York, N.Y.) · 2019Article
- A novel form of RNA double helix based on G·U and C·ARNA (New York, N.Y.) · 2018Article
- Structure of RNA Stem Loop B from the Picornavirus Replication Platform.Biochemistry · 2017Article
- Evidence That Antibiotics Bind to Human Mitochondrial Ribosomal RNA Has Implications for Aminoglycoside Toxicity.The Journal of biological chemistry · 2015Article
- Post-crystallization Improvement of RNA Crystal Diffraction Quality.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Crystal structure studies of RNA duplexes containing s(2)U:A and s(2)U:U base pairs.Journal of the American Chemical Society · 2014Article
- Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.Structure (London, England : 1993) · 2014Article
- Article
- An innate twist between Crick's wobble and Watson-Crick base pairs.RNA (New York, N.Y.) · 2013Review
- The steric hypothesis for DNA replication and fluorine hydrogen bonding revisited in light of structural data.Accounts of chemical research · 2012Review
- Testing the nearest neighbor model for canonical RNA base pairs: revision of GU parameters.Biochemistry · 2012Article
- Crystal structure analysis reveals functional flexibility in the selenocysteine-specific tRNA from mouse.PloS one · 2011Article
- Aromatic N versus aromatic F: bioisosterism discovered in RNA base pairing interactions leads to a novel class of universal base analogs.Nucleic acids research · 2010Article
- Escherichia coli tRNA(Arg) acceptor-stem isoacceptors: comparative crystallization and preliminary X-ray diffraction analysis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Enhancement of the seed-target recognition step in RNA silencing by a PIWI/MID domain protein.Molecular cell · 2009Article
- X-ray diffraction analysis of a human tRNA(Gly) acceptor-stem microhelix isoacceptor at 1.18 A resolution.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
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4 authors.
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Abstract
The acceptor stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALAwt), contains the main identity element for the correct aminoacylation by the alanyl tRNA synthetase. The presence of a G3.U70 wobble base pair is essential for the specificity of this reaction, but there is a debate whether direct minor-groove contact with the 2-amino group of G3 or a distortion of the acceptor stem induced by the wobble pair is the critical feature recognized by the synthetase. We here report the structure analysis of ALAwt at near-atomic resolution using twinned crystals. The crystal lattice is stabilized by a novel strontium binding motif between two cis-diolic O3'-terminal riboses. The two independent molecules in the asymmetric unit of the crystal show overall A-RNA geometry. A comparison with the crystal structure of the G3-C70 mutant of the acceptor stem (ALA(C70)) determined at 1.4 A exhibits a modulation in ALAwt of helical twist and slide due to the wobble base pair, but no recognizable distortion of the helix fragment distant from the wobble base pair. We suggest that a highly conserved hydration pattern in both grooves around the G3.U70 wobble base pair may be functionally significant.
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