ArticleThe Journal of physiology1999
Maintenance and regulation of the pH microclimate at the luminal surface of the distal colon of guinea-pig.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- In vivo Predictive Dissolution Test Using Biorelevant Bicarbonate Buffer for High-dose Free Acid Drug.The AAPS journal · 2026Article
- Interfacial pH Behavior at a Cell/Gate Insulator Nanogap Induced by Allergic Responses.ACS omega · 2019Article
- Somato-dendritic decoupling as a novel mechanism for protracted cortical maturation.BMC biology · 2016Article
- Fluorescent imaging reports an extracellular alkalinization induced by glutamatergic activation of isolated retinal horizontal cells.Journal of neurophysiology · 2014Article
- Cellular Uptake and Intracellular Cargo Release From Dextran Based Nanogel Drug Carriers.Journal of nanotechnology in engineering and medicine · 2013Article
- Electrolyte and acid-base disorders in inflammatory bowel disease.Annals of gastroenterology · 2013Review
- Extracellular pH regulates zinc signaling via an Asp residue of the zinc-sensing receptor (ZnR/GPR39).The Journal of biological chemistry · 2012Article
- Acidification of the synaptic cleft of cone photoreceptor terminal controls the amount of transmitter release, thereby forming the receptive field surround in the vertebrate retina.The journal of physiological sciences : JPS · 2012Review
- Extracellular pH dynamics of retinal horizontal cells examined using electrochemical and fluorometric methods.Journal of neurophysiology · 2012Article
- Decoding epithelial signals: critical role for the epidermal growth factor receptor in controlling intestinal transport function.Acta physiologica (Oxford, England) · 2009Review
- Acetylation of the Entamoeba histone H4 N-terminal domain is influenced by short-chain fatty acids that enter trophozoites in a pH-dependent manner.International journal for parasitology · 2008Article
- Depolarization of isolated horizontal cells of fish acidifies their immediate surrounding by activating V-ATPase.The Journal of physiology · 2007Article
- pH gradients and a micro-pore filter at the luminal surface affect fluxes of propionic acid across guinea pig large intestine.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- Absorption of short-chain fatty acids, sodium and water from the forestomach of camels.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- Propionate absorption associated with bicarbonate secretion in vitro in the mouse cecum.Pflugers Archiv : European journal of physiology · 2007Article
- The effects of dietary phosphorus deficiency on surface pH and membrane composition of the mucosa epithelium in caprine jejunum.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- cAMP regulates plasma membrane vacuolar-type H+-ATPase assembly and activity in blowfly salivary glands.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon.The Journal of physiology · 2005Article
- Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents.World journal of gastroenterology · 2005Article
- Differential Cl- and HCO3- mediated anion secretion by different colonic cell types in response to tetromethylpyrazine.World journal of gastroenterology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The fluorescent dye 5-N-hexadecanoyl-aminofluorescein (HAF) was used to study the mechanisms involved in maintaining a relatively constant luminal surface pH (pHs) in the distal colon of the guinea-pig. The fatty acyl chain of the HAF molecule inserts into the apical membrane of epithelial cells. This allows a continuous measurement of the surface pH for several hours. 2. The localization of HAF was confirmed by confocal laser-scanning microscopy and by using monoclonal antibodies against fluorescein. The insertion of HAF into the apical membrane of the colonocytes did not change the transepithelial conductance or the short-circuit current of the epithelium. 3. With the HAF method a pH microclimate was confirmed at the colonic surface. Although the pH of the bulk luminal solution was decreased in bicarbonate-containing solution from 7.4 to 6.4 the pHs changed only in the range 7.54-6.98. 4. In the absence of bicarbonate pHs almost followed changes of bulk luminal pH. In the presence of bicarbonate there was a decrease in pHs after removal of chloride from the luminal side and an increase in pHs after addition of butyrate to the luminal solution. This suggests the involvement of a bicarbonate-anion exchange in bicarbonate secretion: a Cl--HCO3- as well as a short-chain fatty acid--HCO3- exchange. 5. The apical K+-H+-ATPase in the distal colon of guinea-pig has little influence on pHs in the presence of physiological buffer concentrations. 6. Our findings indicate that bicarbonate plays a major role in maintaining the pH microclimate at the colonic surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.