ArticleNature biotechnology1999
Surface display of a parasite antigen in the ciliate Tetrahymena thermophila.
Article in Nature biotechnology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 80 citations in OpenAlex.
- Development of a Tetrahymena thermophila-Based Vaccine Expressing Miamiensis avidus Ciliary Proteins to Combat Scuticociliatosis.Journal of fish diseases · 2025Article
- Comparative genomics of two protozoansHeliyon · 2024Article
- Target Protein Expression on Tetrahymena thermophila Cell Surface Using the Signal Peptide and GPI Anchor Sequences of the Immobilization Antigen of Cryptocaryon irritans.Molecular biotechnology · 2024Article
- LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena.PLoS genetics · 2019Article
- Functional Proteomics of Nuclear Proteins inGenes · 2019Review
- Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateGenetics · 2019Article
- Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers.Scientific reports · 2019Article
- System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila.The Journal of veterinary medical science · 2018Article
- Article
- ADF/cofilin is not essential but is critically important for actin activities during phagocytosis in Tetrahymena thermophila.Eukaryotic cell · 2013Article
- Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.Methods in enzymology · 2013Article
- Article
- Expression, secretion and surface display of a human alkaline phosphatase by the ciliate Tetrahymena thermophila.BMC biotechnology · 2011Article
- MEC-17 is an alpha-tubulin acetyltransferase.Nature · 2010Article
- Production of recombinant proteins from protozoan parasites.Trends in parasitology · 2010Review
- Transcriptome analysis of the Cryptocaryon irritans tomont stage identifies potential genes for the detection and control of cryptocaryonosis.BMC genomics · 2010Article
- DYF-1 Is required for assembly of the axoneme in Tetrahymena thermophila.Eukaryotic cell · 2009Article
- Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila.Eukaryotic cell · 2008Article
- Comparative studies on the immunogenicity of theronts, tomonts and trophonts of Cryptocaryon irritans in grouper.Parasitology research · 2008Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The ciliated protozoan, Tetrahymena thermophila, offers an attractive medium for the expression of heterologous proteins and could prove particularly useful for the display of foreign proteins on the cell surface. Although progress has been made in transformation of Tetrahymena with heterologous DNA, methods that permit reliable expression of foreign genes have been lacking. Using a mutant strain of T. thermophila carrying a negatively selectable allele of a beta-tubulin gene, we have been able to direct foreign genes to this locus by homologous recombination. Transformed cell lines producing foreign proteins were readily identified and, in at least one case, targeting of proteins to the plasma membrane was accomplished.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.