ArticleThe Plant cell1999
Identification of cis-acting RNA leader elements required for chloroplast psbD gene expression in Chlamydomonas.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 116 citations in OpenAlex.
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Modeling the effect of rRNA-mRNA interactions and mRNA folding on mRNA translation in chloroplasts.Computational and structural biotechnology journal · 2022Article
- Unique Shine-Dalgarno Sequences in Cyanobacteria and Chloroplasts Reveal Evolutionary Differences in Their Translation Initiation.Genome biology and evolution · 2019Article
- Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco.The Plant cell · 2017Article
- Toward mosquito control with a green alga: Expression of Cry toxins ofJournal of applied phycology · 2017Article
- Functional Characterization of a Missing Branch Component inFrontiers in plant science · 2017Article
- A Nucleus-Encoded Chloroplast Phosphoprotein Governs Expression of the Photosystem I Subunit PsaC in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- A nucleus-encoded chloroplast protein regulated by iron availability governs expression of the photosystem I subunit PsaA in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression.The Plant journal : for cell and molecular biology · 2014Article
- The promoter and the 5'-untranslated region of rice metallothionein OsMT2b gene are capable of directing high-level gene expression in germinated rice embryos.Plant cell reports · 2014Article
- Small RNAs reveal two target sites of the RNA-maturation factor Mbb1 in the chloroplast of Chlamydomonas.Nucleic acids research · 2014Article
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Reciprocal regulation of protein synthesis and carbon metabolism for thylakoid membrane biogenesis.PLoS biology · 2013Article
- Availability of Rubisco small subunit up-regulates the transcript levels of large subunit for stoichiometric assembly of its holoenzyme in rice.Plant physiology · 2012Article
- Chloroplast RNase J compensates for inefficient transcription termination by removal of antisense RNA.RNA (New York, N.Y.) · 2011Article
- Light-dependent attenuation of phycoerythrin gene expression reveals convergent evolution of green light sensing in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Function of chloroplast RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2011Review
- The nucleus-encoded trans-acting factor MCA1 plays a critical role in the regulation of cytochrome f synthesis in Chlamydomonas chloroplasts.The Plant cell · 2011Article
- Red light and calmodulin regulate the expression of the psbA binding protein genes in Chlamydomonas reinhardtii.Plant & cell physiology · 2010Article
- Site-specific binding of a PPR protein defines and stabilizes 5' and 3' mRNA termini in chloroplasts.The EMBO journal · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The psbD mRNA of Chlamydomonas reinhardtii is one of the most abundant chloroplast transcripts and encodes the photosystem II reaction center polypeptide D2. This RNA exists in two forms with 5' untranslated regions of 74 and 47 nucleotides. The shorter form, which is associated with polysomes, is likely to result from processing of the larger RNA. Using site-directed mutagenesis and biolistic transformation, we have identified two major RNA stability determinants within the first 12 nucleotides at the 5' end and near position -30 relative to the AUG initiation codon of psbD. Insertion of a polyguanosine tract at position -60 did not appreciably interfere with translation of psbD mRNA. The same poly(G) insertion in the nac2-26 mutant, which is known to be deficient in psbD mRNA accumulation, stabilized the psbD RNA. However, the shorter psbD RNA did not accumulate, and the other psbD RNAs were not translated. Two other elements were found to affect translation but not RNA stability. The first comprises a highly U-rich sequence (positions -20 to -15), and the second, called PRB1 (positions -14 to -11), is complementary to the 3' end of the 16S rRNA. Changing the PRB1 sequence from GGAG to AAAG had no detectable effect on psbD mRNA translation. However, changing this sequence to CCUC led to a fourfold diminished rate of D2 synthesis and accumulation. When the psbD initiation codon was changed to AUA or AUU, D2 synthesis was no longer detected, and psbD RNA accumulated to wild-type levels. The singular organization of the psbD 5' untranslated region could play an important role in the control of initiation of psbD mRNA translation.
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