ArticleThe Journal of experimental medicine1999
Expression of the mouse pre-T cell receptor alpha gene is controlled by an upstream region containing a transcriptional enhancer.
Article in The Journal of experimental medicine, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 52 citations in OpenAlex.
- The transcription factor Zfp281 sustains CD4Cellular & molecular immunology · 2020Article
- Genomic profiling of the transcription factor Zfp148 and its impact on the p53 pathway.Scientific reports · 2020Article
- Notch dimerization is required for leukemogenesis and T-cell development.Genes & development · 2010Article
- Expression of transcription factor zinc-binding protein-89 (ZBP-89) is inhibited by inflammatory cytokines.Pathology and laboratory medicine international · 2009Article
- The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.Differentiation; research in biological diversity · 2009Article
- Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A PDGFRA promoter polymorphism, which disrupts the binding of ZNF148, is associated with primitive neuroectodermal tumours and ependymomas.Journal of medical genetics · 2005Article
- The Epstein-Barr virus protein BMRF1 activates gastrin transcription.Journal of virology · 2005Article
- ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.Nucleic acids research · 2003Article
- Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway.Genes & development · 2002Article
- Regulation of epithelial cell growth by ZBP-89: potential relevance in pancreatic cancer.International journal of gastrointestinal cancer · 2002Review
- The upstream enhancer is necessary and sufficient for the expression of the pre-T cell receptor alpha gene in immature T lymphocytes.The Journal of experimental medicine · 2001Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pre-T cell receptor alpha (pTalpha) protein is a critical component of the pre-T cell receptor complex in early thymocytes. The expression of the pTalpha gene is one of the earliest markers of the T cell lineage and occurs exclusively in pre-T cells. To investigate the molecular basis of thymocyte-specific gene expression, we searched for the genomic elements regulating transcription of the mouse pTalpha gene. We now report that expression of the pTalpha gene is primarily controlled by an upstream genomic region, which can drive thymocyte-specific expression of a marker gene in transgenic mice. Within this region, we have identified two specific DNase-hypersensitive sites corresponding to a proximal promoter and an upstream transcriptional enhancer. The pTalpha enhancer appears to function preferentially in pre-T cell lines and binds multiple nuclear factors, including YY1. The enhancer also contains two G-rich stretches homologous to a critical region of the thymocyte-specific lck proximal promoter. Here we show that these sites bind a common nuclear factor and identify it as the zinc finger protein ZBP-89. Our data establish a novel experimental model for thymocyte-specific gene expression and suggest an important role for ZBP-89 in T cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.