ArticleThe Journal of cell biology1999
PKCepsilon, via its regulatory domain and independently of its catalytic domain, induces neurite-like processes in neuroblastoma cells.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 122 citations in OpenAlex.
- The ε-Isozyme of Protein Kinase C (PKCε) Is Impaired in ALS Motor Cortex and Its Pulse Activation by Bryostatin-1 Produces Long Term Survival in Degenerating SOD1-G93A Motor Neuron-like Cells.International journal of molecular sciences · 2023Article
- Combinatorial molecule screening identified a novel diterpene and the BET inhibitor CPI-203 as differentiation inducers of primary acute myeloid leukemia cells.Haematologica · 2021Article
- Evolving mechanisms of vascular smooth muscle contraction highlight key targets in vascular disease.Biochemical pharmacology · 2018Review
- Protein Kinase C-epsilon in Membrane Delivery during Phagocytosis.Journal of immunological sciences · 2018Article
- Golgi-Associated Protein Kinase C-ε Is Delivered to Phagocytic Cups: Role of Phosphatidylinositol 4-Phosphate.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Protein Kinase C as Regulator of Vascular Smooth Muscle Function and Potential Target in Vascular Disorders.Advances in pharmacology (San Diego, Calif.) · 2017Review
- Protein Kinase Cϵ (PKCϵ) Promotes Synaptogenesis through Membrane Accumulation of the Postsynaptic Density Protein PSD-95.The Journal of biological chemistry · 2016Article
- Molecular characterization of protein kinase C delta (PKCδ)-Smac interactions.BMC biochemistry · 2016Article
- Orexin A inhibits propofol-induced neurite retraction by a phospholipase D/protein kinase Cε-dependent mechanism in neurons.PloS one · 2014Article
- PKC-ε pseudosubstrate and catalytic activity are necessary for membrane delivery during IgG-mediated phagocytosis.Journal of leukocyte biology · 2013Article
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- Direct binding of RalA to PKCη and its crucial role in morphological change during keratinocyte differentiation.Molecular biology of the cell · 2011Article
- The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases.Frontiers of medicine · 2011Review
- C1 Domain-targeted isophthalate derivatives induce cell elongation and cell cycle arrest in HeLa cells.PloS one · 2011Article
- Protein kinase C-{delta} regulates the subcellular localization of Shc in H2O2-treated cardiomyocytes.American journal of physiology. Cell physiology · 2010Article
- PKCalpha expression is a marker for breast cancer aggressiveness.Molecular cancer · 2010Article
- Protein kinase Cdelta supports survival of MDA-MB-231 breast cancer cells by suppressing the ERK1/2 pathway.The Journal of biological chemistry · 2009Article
- Phosphoinositide and inositol phosphate analysis in lymphocyte activation.Current protocols in immunology · 2009Article
- Rationally designed peptide regulators of protein kinase C.Trends in endocrinology and metabolism: TEM · 2009Review
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the role of protein kinase C (PKC) isoforms in regulation of neurite outgrowth, PKCalpha, betaII, delta, and epsilon fused to enhanced green fluorescent protein (EGFP) were transiently overexpressed in neuroblastoma cells. Overexpression of PKCepsilon-EGFP induced cell processes whereas the other isoforms did not. The effect of PKCepsilon-EGFP was not suppressed by the PKC inhibitor GF109203X. Instead, process formation was more pronounced when the regulatory domain was introduced. Overexpression of various fragments from PKCepsilon regulatory domain revealed that a region encompassing the pseudosubstrate, the two C1 domains, and parts of the V3 region were necessary and sufficient for induction of processes. By deleting the second C1 domain from this construct, a dominant-negative protein was generated which suppressed processes induced by full-length PKCepsilon and neurites induced during retinoic acid- and growth factor-induced differentiation. As with neurites in differentiated neuroblastoma cells, processes induced by the PKCepsilon- PSC1V3 protein contained alpha-tubulin, neurofilament-160, and F-actin, but the PKCepsilon-PSC1V3-induced processes lacked the synaptic markers synaptophysin and neuropeptide Y. These data suggest that PKCepsilon, through its regulatory domain, can induce immature neurite-like processes via a mechanism that appears to be of importance for neurite outgrowth during neuronal differentiation.
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