ArticleMolecular and cellular biology1999
Regulation of AUF1 expression via conserved alternatively spliced elements in the 3' untranslated region.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Isoforms in action: The impact of transcript diversity in phenotype.Genetics and molecular biology · 2026Article
- Coupling of alternative splicing and alternative polyadenylation.Acta biochimica et biophysica Sinica · 2024Review
- 3'UTR Diversity: Expanding Repertoire of RNA Alterations in Human mRNAs.Molecules and cells · 2023Review
- Reduction of integrin alpha 4 activity through splice modulating antisense oligonucleotides.Scientific reports · 2019Article
- Auto- and cross-regulation of the hnRNPs D and DL.RNA (New York, N.Y.) · 2018Article
- RNA Degradation in Neurodegenerative Disease.Advances in neurobiology · 2018Review
- Control of gene expression through the nonsense-mediated RNA decay pathway.Cell & bioscience · 2017Review
- Alternative splicing mechanisms orchestrating post-transcriptional gene expression: intron retention and the intron-rich genome of apicomplexan parasites.Current genetics · 2016Article
- HuR is required for IL-17-induced Act1-mediated CXCL1 and CXCL5 mRNA stabilization.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- MicroRNA regulation of T-lymphocyte immunity: modulation of molecular networks responsible for T-cell activation, differentiation, and development.Critical reviews in immunology · 2013Review
- Expression of two novel alternatively spliced COL2A1 isoforms during chondrocyte differentiation.Matrix biology : journal of the International Society for Matrix Biology · 2008Article
- Analysis of turnover and translation regulatory RNA-binding protein expression through binding to cognate mRNAs.Molecular and cellular biology · 2007Article
- Upf1/Upf2 regulation of 3' untranslated region splice variants of AUF1 links nonsense-mediated and A+U-rich element-mediated mRNA decay.Molecular and cellular biology · 2006Article
- hUPF2 silencing identifies physiologic substrates of mammalian nonsense-mediated mRNA decay.Molecular and cellular biology · 2006Article
- A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay.BMC molecular biology · 2005Article
- Regulation of the MID1 protein function is fine-tuned by a complex pattern of alternative splicing.Human genetics · 2004Article
- An unappreciated role for RNA surveillance.Genome biology · 2004Article
- Nitric oxide increases the decay of matrix metalloproteinase 9 mRNA by inhibiting the expression of mRNA-stabilizing factor HuR.Molecular and cellular biology · 2003Article
- A+U-rich-element RNA-binding factor 1/heterogeneous nuclear ribonucleoprotein D gene expression is regulated by oestrogen in the rat uterus.The Biochemical journal · 2002Article
- The role of 3'-untranslated region (3'-UTR) mediated mRNA stability in cardiovascular pathophysiology.Molecular and cellular biochemistry · 2001Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The A+U-rich RNA-binding factor AUF1 exhibits characteristics of a trans-acting factor contributing to the rapid turnover of many cellular mRNAs. Structural mapping of the AUF1 gene and its transcribed mRNA has revealed alternative splicing events within the 3' untranslated region (3'-UTR). In K562 erythroleukemia cells, we have identified four alternatively spliced AUF1 3'-UTR variants, including a population of AUF1 mRNA containing a highly conserved 107-nucleotide (nt) 3'-UTR exon (exon 9) and the adjacent downstream intron (intron 9). Functional analyses using luciferase-AUF1 3'-UTR chimeric transcripts demonstrated that the presence of either a spliceable or an unspliceable intron 9 in the 3'-UTR repressed luciferase expression in cis, indicating that intron 9 sequences may down-regulate gene expression by two distinct mechanisms. In the case of the unspliceable intron, repression of luciferase expression likely involved two AUF1-binding sequences, since luciferase expression was increased by deletion of these sites. However, inclusion of the spliceable intron in the luciferase 3'-UTR down-regulated expression independent of the AUF1-binding sequences. This is likely due to nonsense-mediated mRNA decay (NMD) owing to the generation of exon-exon junctions more than 50 nt downstream of the luciferase termination codon. AUF1 mRNA splice variants generated by selective excision of intron 9 are thus also likely to be subject to NMD since intron 9 is always positioned >137 nt downstream of the stop codon. The distribution of alternatively spliced AUF1 transcripts in K562 cells is consistent with this model of regulated AUF1 expression.
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