ArticleGenome research1999
Fluorescence polarization in homogeneous nucleic acid analysis.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
145 citing papers in PubMed, 1 synthesis or guideline pooled it, 394 citations in OpenAlex.
- Evaluation of a susceptibility gene for schizophrenia: genotype based meta-analysis of RGS4 polymorphisms from thirteen independent samples.Biological psychiatry · 2006Pooled it
- Association of FKBP5 polymorphisms with suicidal events in the Treatment of Resistant Depression in Adolescents (TORDIA) study.The American journal of psychiatry · 2010Trial
- Haplotypic association spanning the 22q11.21 genes COMT and ARVCF with schizophrenia.Molecular psychiatry · 2005Trial
- Functional interferon-epsilon gene polymorphisms and sexually transmitted infections of the endometrium.American journal of reproductive immunology (New York, N.Y. : 1989) · 2023Article
- FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance-dependent energy transfer.Protein science : a publication of the Protein Society · 2023Article
- Nanomaterials Used in Fluorescence Polarization Based Biosensors.International journal of molecular sciences · 2022Review
- Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood-brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study.Critical care (London, England) · 2021Observational
- Practical genotyping by single-nucleotide primer extension.Biology methods & protocols · 2020Article
- Circulating Interleukin-6 concentration covaries inversely with self-reported sleep duration as a function of polymorphic variation in the glucocorticoid receptor.Brain, behavior, and immunity · 2019Article
- Improving office workers' mental health and cognition: a 3-arm cluster randomized controlled trial targeting physical activity and sedentary behavior in multi-component interventions.BMC public health · 2019Article
- Article
- Genotype-based recall to study metabolic effects of genetic variation: a pilot study of PPARG Pro12Ala carriers.Upsala journal of medical sciences · 2017Article
- xMAP Technology: Applications in Detection of Pathogens.Frontiers in microbiology · 2017Review
- Association between polymorphisms of APE1 and OGG1 and risk of colorectal cancer in Taiwan.World journal of gastroenterology · 2016Article
- Article
- Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- OXTR polymorphism predicts social relationships through its effects on social temperament.Social cognitive and affective neuroscience · 2015Article
- Genetic polymorphisms in DNA repair and oxidative stress pathways may modify the association between body size and postmenopausal breast cancer.Annals of epidemiology · 2015Article
- COMT met allele differentially predicts risk versus severity of aberrant eating in a large community sample.Psychiatry research · 2014Article
- The BDNF Val66Met polymorphism does not moderate the effect of self-reported physical activity on depressive symptoms in midlife.Psychiatry research · 2014Article
85 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence polarization is described. In this method, amplified genomic DNA containing a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DNA template adjacent to the polymorphic site) in the presence of allele-specific dye-labeled dideoxyribonucleoside triphosphates and a commercially available modified Taq DNA polymerase. The primer is extended by the dye-terminator specific for the allele present on the template, increasing approximately 10-fold the molecular weight of the fluorophore. At the end of the reaction, the fluorescence polarization of the two dye-terminators in the reaction mixture are analyzed directly without separation or purification. This homogeneous DNA diagnostic method is shown to be highly sensitive and specific and is suitable for automated genotyping of large number of samples. [The data shown in Figure 3 are available as an online supplement at.]
Indexed as
Identifiers
10330129PMC310763W2160437131What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.