ArticleThe EMBO journal1999
Transcription of the human U2 snRNA genes continues beyond the 3' box in vivo.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach.Nature communications · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- SUMO conjugation regulates the activity of the Integrator complex.Nucleic acids research · 2022Article
- Alternative splicing of DSP1 enhances snRNA accumulation by promoting transcription termination and recycle of the processing complex.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Regulation of expression of human RNA polymerase II-transcribed snRNA genes.Open biology · 2017Review
- A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II.Journal of cell science · 2014Article
- Human snRNA genes use polyadenylation factors to promote efficient transcription termination.Nucleic acids research · 2014Article
- The little elongation complex functions at initiation and elongation phases of snRNA gene transcription.Molecular cell · 2013Article
- The 5S rDNA high dynamism in Diplodus sargus is a transposon-mediated mechanism. Comparison with other multigene families and Sparidae species.Journal of molecular evolution · 2013Article
- Evidence for 5S rDNA horizontal transfer in the toadfish Halobatrachus didactylus (Schneider, 1801) based on the analysis of three multigene families.BMC evolutionary biology · 2012Article
- Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.Molecular cell · 2012Article
- Genomic study of RNA polymerase II and III SNAPc-bound promoters reveals a gene transcribed by both enzymes and a broad use of common activators.PLoS genetics · 2012Article
- In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.PloS one · 2012Article
- The linked units of 5S rDNA and U1 snDNA of razor shells (Mollusca: Bivalvia: Pharidae).Heredity · 2011Article
- snRNA 3' end formation: the dawn of the Integrator complex.Biochemical Society transactions · 2010Review
- The scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region.Nucleic acids research · 2010Article
- Chromatin structure is implicated in "late" elongation checkpoints on the U2 snRNA and beta-actin genes.Molecular and cellular biology · 2009Article
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- Human U2 snRNA genes exhibit a persistently open transcriptional state and promoter disassembly at metaphase.Molecular and cellular biology · 2008Article
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Authors and funding
5 authors.
Funding
Abstract
The 3' box of the human class II snRNA genes is required for proper 3' processing of transcripts, but how it functions is unclear. Several lines of evidence suggest that termination of transcription occurs at the 3' box and the terminated transcript is then a substrate for processing. However, using nuclear run-on analysis of endogenous genes, we demonstrate that transcription continues for at least 250 nucleotides beyond the 3' box of the U2 genes. Although in vivo footprinting analysis of both the U1 and U2 genes detects no protein-DNA contacts directly over the 3' box, a series of G residues immediately downstream from the 3' box of the U1 gene are clearly protected from methylation by dimethylsulfate. In conjunction with the 3' box of the U1 gene, this in vivo footprinted region causes termination of transcription of transiently transfected U2 constructs, whereas a 3' box alone does not. Taken together, these results indicate that the 3' box is not an efficient transcriptional terminator but may act as a processing element that is functional in the nascent RNA.
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