ArticleThe EMBO journal1999
TGF-beta cooperates with TGF-alpha to induce the self-renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 97 citations in OpenAlex.
- TopoDoE: a design of experiment strategy for selection and refinement in ensembles of executable gene regulatory networks.BMC bioinformatics · 2024Article
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- Modelling the erythroblastic island niche of dyserythropoietic anaemia type IV patients using induced pluripotent stem cells.Frontiers in cell and developmental biology · 2023Article
- Hypothesis: Low Vitamin A and D Levels Worsen Clinical Outcomes When Children with Sickle Cell Disease Encounter Parvovirus B19.Nutrients · 2022Article
- Evidence for close molecular proximity between reverting and undifferentiated cells.BMC biology · 2022Article
- Practical identifiability in the frame of nonlinear mixed effects models: the example of the in vitro erythropoiesis.BMC bioinformatics · 2021Article
- WASABI: a dynamic iterative framework for gene regulatory network inference.BMC bioinformatics · 2019Article
- Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements.PloS one · 2019Article
- Drugs modulating stochastic gene expression affect the erythroid differentiation process.PloS one · 2019Article
- Calibration, Selection and Identifiability Analysis of a Mathematical Model of the in vitro Erythropoiesis in Normal and Perturbed Contexts.In silico biology · 2019Article
- Automated cell cycle and cell size measurements for single-cell gene expression studies.BMC research notes · 2018Article
- SINCERITIES: inferring gene regulatory networks from time-stamped single cell transcriptional expression profiles.Bioinformatics (Oxford, England) · 2018Article
- Hematopoietic Cytokine Gene Duplication in Zebrafish Erythroid and Myeloid Lineages.Frontiers in cell and developmental biology · 2018Review
- Single-Cell-Based Analysis Highlights a Surge in Cell-to-Cell Molecular Variability Preceding Irreversible Commitment in a Differentiation Process.PLoS biology · 2016Article
- RNA-seq Profiles of Immune Related Genes in the Spleen of Necrotic Enteritis-afflicted Chicken Lines.Asian-Australasian journal of animal sciences · 2015Article
- Origins of the Vertebrate Erythro/Megakaryocytic System.BioMed research international · 2015Review
- The role of spatial organization of cells in erythropoiesis.Journal of mathematical biology · 2015Article
- V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors.Oncogene · 2014Article
- Cholesterol synthesis-related enzyme oxidosqualene cyclase is required to maintain self-renewal in primary erythroid progenitors.Cell proliferation · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simultaneous addition of both TGF-alpha and TGF-beta induces the sustained, long-term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2-day-old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF-alpha and TGF-beta are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF-alpha and TGF-beta, as well as glucocorticoids. Proliferating, established T2ECs still require TGF-alpha, but are independent of exogenous TGF-beta. Using a TGF-beta-neutralizing antibody or expressing a dominant-negative TGF-beta receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF-beta during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek-1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF-alpha and -beta receptors. These results establish a novel mechanism by which self-renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi-optimal model system to study erythrocytic progenitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.