ArticleJournal of clinical microbiology1999
Quantitative detection of Borrelia burgdorferi by real-time PCR.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Efficacy of RevolutionParasites & vectors · 2026Article
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- TICKHUNTER: A Targeted Hybridization-Capture Sequencing Approach for the Detection and Characterization of Tick-borne Pathogens and Blood Meals.bioRxiv : the preprint server for biology · 2025Article
- Presence of Borrelia Spirochetes in White Stork (Animals : an open access journal from MDPI · 2024Article
- RecombinantPathogens (Basel, Switzerland) · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.PLoS pathogens · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.bioRxiv : the preprint server for biology · 2024Article
- Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection.Scientific reports · 2024Article
- Development of a Multiplex qPCR Assay for Fast Detection and Differentiation of Paracoccidioidomycosis Agents.Journal of fungi (Basel, Switzerland) · 2023Article
- Genomic hybrid capture assay to detectJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Trends in Molecular Diagnostics and Genotyping Tools Applied for EmergingJournal of fungi (Basel, Switzerland) · 2022Review
- Pathogenesis of Borrelia burgdorferi and Babesia microti in TLR4-Competent and TLR4-dysfunctional C3H mice.Cellular microbiology · 2021Article
- A New Duplex PCR-Assay for the Detection and Identification ofJournal of fungi (Basel, Switzerland) · 2021Article
- Specific composition of polyphenolic compounds with fatty acids as an approach in helping to reduce spirochete burden in Lyme disease:Therapeutic advances in chronic disease · 2020Article
- Prevention of transmission of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum by Ixodes spp. ticks to dogs treated with the Seresto® collar (imidacloprid 10% + flumethrin 4.5%).Parasitology research · 2020Article
- Borrelia burgdorferi chemotaxis toward tick protein Salp12 contributes to acquisition.Ticks and tick-borne diseases · 2019Article
- Neuroborreliosis in a horse with common variable immunodeficiency.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR.Journal of visualized experiments : JoVE · 2018Article
- An Immunosuppressive Tick Salivary Gland Protein DsCystatin Interferes With Toll-Like Receptor Signaling by Downregulating TRAF6.Frontiers in immunology · 2018Article
- Dectin-1 Positive Dendritic Cells Expand after Infection withFrontiers in immunology · 2018Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, no easy and reliable methods allowing for the quantification of Borrelia burgdorferi in tissues of infected humans or animals are available. Due to the lack of suitable assays to detect B. burgdorferi CFU and the qualitative nature of the currently performed PCR assays, we decided to exploit the recently developed real-time PCR. This technology measures the release of fluorescent oligonucleotides during the PCR. Flagellin of B. burgdorferi was chosen as the target sequence. A linear quantitative detection range of 5 logs with a calculated detection limit of one to three spirochetes per assay reaction mixture was observed. The fact that no signals were obtained with closely related organisms such as Borrelia hermsii argues for a high specificity of this newly developed method. A similar method was developed to quantify mouse actin genomic sequences to allow for the standardization of spirochete load. The specificity and sensitivity of the B. burgdorferi and the actin real-time PCR were not altered when samples were spiked with mouse cells or spirochetes, respectively. To evaluate the applicability of the real-time PCR, we used the mouse model of Lyme disease. The fate of B. burgdorferi was monitored in different tissues from inbred mice and from mice treated with antibiotics. Susceptible C3H/HeJ mice had markedly higher burdens of bacterial DNA than resistant BALB/c mice, and penicillin G treatment significantly reduced the numbers of spirochetes. Since these results show a close correlation between clinical symptoms and bacterial burden of tissues, we are currently analyzing human biopsy specimens to evaluate the real-time PCR in a diagnostic setting.
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