ArticleJournal of clinical microbiology1999
PCR-restriction enzyme analysis for detection of Candida DNA in blood from febrile patients with hematological malignancies.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Biomarker-guided antifungal stewardship policies for patients with invasive candidiasis.Current medical mycology · 2018Review
- Application of Culture-Independent Rapid Diagnostic Tests in the Management of Invasive Candidiasis and Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2015Review
- Neonatal candidiasis: diagnosis, prevention, and treatment.The Journal of infection · 2014Review
- Invasive fungal infections in patients with cancer in the Intensive Care Unit.International journal of antimicrobial agents · 2012Review
- In Vitro Antifungal Activity of Ankaferd Blood Stopper Against Candida albicans.Current therapeutic research, clinical and experimental · 2011Article
- Canadian clinical practice guidelines for invasive candidiasis in adults.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2010Article
- PCR-based diagnosis of human fungal infections.Expert review of anti-infective therapy · 2009Review
- Bench-to-bedside review: Candida infections in the intensive care unit.Critical care (London, England) · 2008Review
- High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray.Journal of clinical microbiology · 2006Article
- Comparison of non-culture-based methods for detection of systemic fungal infections, with an emphasis on invasive Candida infections.Journal of clinical microbiology · 2005Article
- A prospective study of real-time panfungal PCR for the early diagnosis of invasive fungal infection in haemato-oncology patients.Bone marrow transplantation · 2005Article
- The diagnosis of hepatosplenic candidiasis by DNA analysis of tissue biopsy and serum.Journal of clinical pathology · 2004Article
- Combination antifungal therapy.Antimicrobial agents and chemotherapy · 2004Review
- Rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2003Article
- Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.Folia microbiologica · 2003Article
- Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR.Journal of clinical microbiology · 2002Article
- Current status of nonculture methods for diagnosis of invasive fungal infections.Clinical microbiology reviews · 2002Review
- Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.Journal of clinical microbiology · 2002Article
- Rapid identification of yeasts in positive blood cultures by a multiplex PCR method.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood samples were drawn daily from 72 patients who had hematological malignancies, neutropenia, and fever and who had failed to respond to broad-spectrum antibiotics. Each sample was used for conventional fungal blood cultures and for detection and identification of Candida DNA by a PCR method with subsequent restriction enzyme analysis (REA) recently developed in our laboratory. The PCR method was able to detect five CFU of Candida spp. per ml of blood, and subsequent REA of the amplicons allowed the identification of the Candida species most commonly implicated in cases of candidiasis. Thirty-one patients were PCR-REA positive, and four of these patients were also culture positive. The ultimate diagnosis for 13 of these patients and 1 patient who was PCR-REA negative was disseminated candidiasis (confirmed by clinical data, multiple cultures, histology, autopsy, and/or ultrasonographic evidence of hepatosplenic candidiasis). The molecular method is significantly more sensitive than conventional fungal blood cultures and has a high negative predictive value (97.5%) for the development of disseminated candidiasis in neutropenic patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.