ArticleJournal of clinical microbiology1999
Use of PCR in diagnosis of human american tegumentary leishmaniasis in Rio de Janeiro, Brazil.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 137 citations in OpenAlex.
- Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis.Memorias do Instituto Oswaldo Cruz · 2015Pooled it
- Trial
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- Molecular Detection ofAdvanced biomedical research · 2026Article
- Clinical and parasitological features of Leishmania infection among gold miners in the Oiapoque basin, an international Brazil-French Guiana border.PLoS neglected tropical diseases · 2024Article
- Evaluation of Molecular Methods to Identify Chagas Disease and Leishmaniasis in Blood Donation Candidates in Two Brazilian Centers.Pathogens (Basel, Switzerland) · 2023Article
- A critical review of the applicability of serological screening for Leishmaniasis in blood banks in Brazil.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Review
- Article
- Demographical and population dynamics impact on public health of cutaneousAfrican health sciences · 2019Article
- Species typing in dermal leishmaniasis.Clinical microbiology reviews · 2015Review
- Severity of tegumentary leishmaniasis is not exclusively associated with Leishmania RNA virus 1 infection in Brazil.Memorias do Instituto Oswaldo Cruz · 2013Article
- Consecutive cutaneous and visceral leishmaniasis manifestations involving a novel Leishmania species in two HIV patients in Thailand.The American journal of tropical medicine and hygiene · 2012Article
- SYBR Green-based real-time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases.Parasites & vectors · 2012Article
- Mucosal Leishmaniasis caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon.PLoS neglected tropical diseases · 2011Article
- Article
- Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis.Journal of clinical laboratory analysis · 2009Article
- Mucocutaneous leishmaniasis: an imported infection among travellers to central and South America.BMJ (Clinical research ed.) · 2004Article
- Comparative analysis of the nitric oxide production by Leishmania sp.Medical microbiology and immunology · 2003Article
- Prospective value of PCR amplification and sequencing for diagnosis and typing of old world Leishmania infections in an area of nonendemicity.Journal of clinical microbiology · 2003Article
- Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris).Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania (Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool for the diagnosis of leishmaniasis. Biopsy specimens from cutaneous or mucosal lesions were taken from 230 individuals from areas where Leishmania is endemic: 216 patients who had a clinical picture suggestive of leishmaniasis and 14 individuals with cutaneous lesions due to other causes. Each specimen was processed for histopathologic examination, culture, touch preparation, and DNA isolation. Oligonucleotides that amplify the conserved region of the minicircle molecules of Leishmania were used in a hot-start PCR. While at least one conventional technique was positive for Leishmania for 62% (134 of 216) of the patients, PCR coupled to hybridization was positive for 94% (203 of 216) of the patients. The 14 patients whose clinical picture was not suggestive of leishmaniasis had negative results by all techniques. The impact of the PCR was striking in mucosal disease. While the disease in only 17% (4 of 24) of the patients could be diagnosed by conventional techniques, PCR was positive for 71% (17 of 24) of the patients. Hybridization showed that all cases of disease were caused by parasites belonging to the Viannia subgenus. Altogether, the results indicate that PCR is a valuable tool for the diagnosis of leishmaniasis on a routine basis and is likely to provide valuable epidemiological information about the disease in countries where it is endemic.
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