ArticleGenes & development1999
Cyclin-dependent kinase and Cks/Suc1 interact with the proteasome in yeast to control proteolysis of M-phase targets.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 82 citations in OpenAlex.
- Anaphase onset requires CKS-1-mediated destruction of securin in meiosis I and cyclin B1 in meiosis II.The Journal of cell biology · 2026Article
- Cks overexpression enhances chemotherapeutic efficacy by overriding DNA damage checkpoints.Oncogene · 2015Article
- Silencing of KIF14 interferes with cell cycle progression and cytokinesis by blocking the p27(Kip1) ubiquitination pathway in hepatocellular carcinoma.Experimental & molecular medicine · 2014Article
- Cks1 enhances transcription efficiency at the GAL1 locus by linking the Paf1 complex to the 19S proteasome.Eukaryotic cell · 2013Article
- New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.G3 (Bethesda, Md.) · 2012Article
- State of the APC/C: organization, function, and structure.Critical reviews in biochemistry and molecular biology · 2011Review
- Discovering pathways by orienting edges in protein interaction networks.Nucleic acids research · 2011Article
- Structure characterization of the 26S proteasome.Biochimica et biophysica acta · 2011Review
- Cks1, Cdk1, and the 19S proteasome collaborate to regulate gene induction-dependent nucleosome eviction in yeast.Molecular and cellular biology · 2010Article
- Cks1 activates transcription by binding to the ubiquitylated proteasome.Molecular and cellular biology · 2010Article
- An overview of Cdk1-controlled targets and processes.Cell division · 2010Article
- Loss of cks1 homeostasis deregulates cell division cycle.Journal of cellular and molecular medicine · 2010Review
- Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase.Cell division · 2009Article
- Bayesian Orthogonal Least Squares (BOLS) algorithm for reverse engineering of gene regulatory networks.BMC bioinformatics · 2007Article
- Mutations in the yeast cyclin-dependent kinase Cdc28 reveal a role in the spindle assembly checkpoint.Molecular genetics and genomics : MGG · 2003Article
- Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4.The EMBO journal · 2001Article
- A role for the Swe1 checkpoint kinase during filamentous growth of Saccharomyces cerevisiae.Genetics · 2001Article
- A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex.Nucleic acids research · 2001Article
- A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1.The Journal of cell biology · 2001Article
- Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Cell cycle-specific proteolysis is critical for proper execution of mitosis in all eukaryotes. Ubiquitination and subsequent proteolysis of the mitotic regulators Clb2 and Pds1 depend on the cyclosome/APC and the 26S proteasome. We report here that components of the cell cycle machinery in yeast, specifically the cell cycle regulatory cyclin-dependent kinase Cdc28 and a conserved associated protein Cks1/Suc1, interact genetically, physically, and functionally with components of the 26S proteasome. A mutation in Cdc28 (cdc28-1N) that interferes with Cks1 binding, or inactivation of Cks1 itself, confers stabilization of Clb2, the principal mitotic B-type cyclin in budding yeast. Surprisingly, Clb2-ubiquitination in vivo and in vitro is not affected by mutations in cks1, indicating that Cks1 is not essential for cyclosome/APC activity. However, mutant Cks1 proteins no longer physically interact with the proteasome, suggesting that Cks1 is required for some aspect of proteasome function during M-phase-specific proteolysis. We further provide evidence that Cks1 function is required for degradation of the anaphase inhibitor Pds1. Stabilization of Pds1 is partially responsible for the metaphase arrest phenotype of cks1 mutants because deletion of PDS1 partially relieves the metaphase block in these mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.