ArticleGenes & development1999
UASrpg can function as a heterochromatin boundary element in yeast.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 129 citations in OpenAlex.
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- H3K56 deacetylation and H2A.Z deposition are required for aberrant heterochromatin spreading.Nucleic acids research · 2022Article
- Mutational analysis identifies functional Rap1, Su(Hw), and CTCF insulator sites in Arabidopsis thaliana.Plant cell reports · 2020Article
- Review
- Design and Construction of Generalizable RNA-Protein Hybrid Controllers by Level-Matched Genetic Signal Amplification.Cell systems · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Chromatin regulation at the frontier of synthetic biology.Nature reviews. Genetics · 2015Review
- Chromosome boundary elements and regulation of heterochromatin spreading.Cellular and molecular life sciences : CMLS · 2014Review
- Nucleosome-positioning sequence repeats impact chromatin silencing in yeast minichromosomes.Genetics · 2014Article
- Uncoupling transcription from covalent histone modification.PLoS genetics · 2014Article
- Detection of an altered heterochromatin structure in the absence of the nucleotide excision repair protein Rad4 in Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2013Article
- Functional analysis of the HS185 regulatory element in the rice HSP70 promoter.Molecular biology reports · 2012Article
- Functions of protosilencers in the formation and maintenance of heterochromatin in Saccharomyces cerevisiae.PloS one · 2012Article
- A protosilencer of subtelomeric gene expression in Candida glabrata with unique properties.Genetics · 2012Article
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Analysis of the enhancer-blocking function of the TBS element from Petunia hybrida in transgenic Arabidopsis thaliana and Nicotiana tabacum.Plant cell reports · 2011Article
- Enhancer-promoter interference and its prevention in transgenic plants.Plant cell reports · 2011Review
- A BEAF dependent chromatin domain boundary separates myoglianin and eyeless genes of Drosophila melanogaster.Nucleic acids research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The HM loci in Saccharomyces cerevisiae constitute region-specific but gene-nonspecific repression domains, as a number of heterologous genes transcribed by RNA polymerase II or III are silenced when placed at these loci. The promoters of the Ashbya gossypii TEF gene and the S. cerevisiae TEF1 and TEF2 genes, however, are resistant to transcriptional silencing by the HM silencers in yeast. Moreover, when interposed between the HML alpha genes and the E silencer, certain segments of these promoters block the repression effect of the silencer on the alpha genes. All of these fragments contain UASrpg (upstream activation sequence of ribosome protein genes) composed of multiple binding sites for Rap1. In fact, a 149-bp segment consisting essentially of only three tandem Rap1-binding sites from the UASrpg of yeast TEF2 exhibits silencer-blocking activity. This element also exhibits insulating activity and orientation dependence characteristic of known chromatin boundary elements. Finally, the element blocks the physical spread of heterochromatin initiated at a silencer. This segment provides the first example of chromatin domain boundary or insulator elements in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.