ArticleJournal of bacteriology1999
The type II pullulanase of Thermococcus hydrothermalis: molecular characterization of the gene and expression of the catalytic domain.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Characterization of protein glycosylation in an Asgard archaeon.BBA advances · 2024Article
- Identification of acacia gum fermenting bacteria from pooled human feces using anaerobic enrichment culture.Frontiers in microbiology · 2023Article
- Identification and Characterization of Dextran α-1,2-Debranching Enzyme fromJournal of applied glycoscience · 2023Article
- Biotechnological applications of archaeal enzymes from extreme environments.Biological research · 2018Review
- In silico analysis of the α-amylase family GH57: eventual subfamilies reflecting enzyme specificities.3 Biotech · 2018Article
- Cohnella amylopullulanases: Biochemical characterization of two recombinant thermophilic enzymes.PloS one · 2017Article
- Structure and function of α-glucan debranching enzymes.Cellular and molecular life sciences : CMLS · 2016Review
- Adaptation of the cecal bacterial microbiome of growing pigs in response to resistant starch type 4.Applied and environmental microbiology · 2015Article
- Membrane Association and Catabolite Repression of the Sulfolobus solfataricus α-Amylase.Microorganisms · 2015Article
- α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.Cellular and molecular life sciences : CMLS · 2014Review
- Novel maltotriose-hydrolyzing thermoacidophilic type III pullulan hydrolase from Thermococcus kodakarensis.Applied and environmental microbiology · 2014Article
- Resistant starch: promise for improving human health.Advances in nutrition (Bethesda, Md.) · 2013Review
- Sequence fingerprints of enzyme specificities from the glycoside hydrolase family GH57.Extremophiles : life under extreme conditions · 2012Article
- Effect of C-terminal truncation on enzyme properties of recombinant amylopullulanase from Thermoanaerobacter pseudoethanolicus.Extremophiles : life under extreme conditions · 2012Article
- A GH57 family amylopullulanase from deep-sea Thermococcus siculi: expression of the gene and characterization of the recombinant enzyme.Current microbiology · 2011Article
- Characterization of ApuB, an extracellular type II amylopullulanase from Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2008Article
- Biochemical characterization of engineered amylopullulanase from Thermoanaerobacter ethanolicus 39E-implicating the non-necessity of its 100 C-terminal amino acid residues.Extremophiles : life under extreme conditions · 2008Article
- Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance.Journal of bacteriology · 2007Article
- A novel branching enzyme of the GH-57 family in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.Journal of bacteriology · 2006Article
- Insights into ABC transport in archaea.Journal of bioenergetics and biomembranes · 2004Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding a hyperthermostable type II pullulanase produced by Thermococcus hydrothermalis (Th-Apu) has been isolated. Analysis of a total of 5.2 kb of genomic DNA has revealed the presence of three open reading frames, one of which (apuA) encodes the pullulanase. This enzyme is composed of 1,339 amino acid residues and exhibits a multidomain structure. In addition to a typical N-terminal signal peptide, Th-Apu possesses a catalytic domain, a domain bearing S-layer homology-like motifs, a Thr-rich region, and a potential C-terminal transmembrane domain. The presence of these noncatalytic domains suggests that Th-Apu may be anchored to the cell surface and be O glycosylated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.