ArticleJournal of bacteriology1999
Septal localization of penicillin-binding protein 1 in Bacillus subtilis.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- RagB stimulates the activity of the peptidoglycan polymerase RodA in Bacillus subtilis.EMBO reports · 2025Article
- GpsB interacts with FtsZ in multiple species and may serve as an accessory Z-ring anchor.Molecular biology of the cell · 2025Article
- Penicillin-binding protein redundancy inApplied and environmental microbiology · 2024Article
- Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands.Cell surface (Amsterdam, Netherlands) · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Cell morphology maintenance in Bacillus subtilis through balanced peptidoglycan synthesis and hydrolysis.Scientific reports · 2020Article
- Harnessing β-Lactam Antibiotics for Illumination of the Activity of Penicillin-Binding Proteins inACS chemical biology · 2020Article
- Article
- Subunit Arrangement in GpsB, a Regulator of Cell Wall Biosynthesis.Microbial drug resistance (Larchmont, N.Y.) · 2016Article
- Lipid-dependent generation of dual topology for a membrane protein.The Journal of biological chemistry · 2012Article
- Selective penicillin-binding protein imaging probes reveal substructure in bacterial cell division.ACS chemical biology · 2012Article
- Article
- Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria.PLoS pathogens · 2010Article
- Characterization of the Synechocystis strain PCC 6803 penicillin-binding proteins and cytokinetic proteins FtsQ and FtsW and their network of interactions with ZipN.Journal of bacteriology · 2009Article
- Cytological characterization of YpsB, a novel component of the Bacillus subtilis divisome.Journal of bacteriology · 2008Article
- Functional characterization and membrane topology of Escherichia coli WecA, a sugar-phosphate transferase initiating the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide.Journal of bacteriology · 2007Article
- Bacterial cell wall synthesis: new insights from localization studies.Microbiology and molecular biology reviews : MMBR · 2005Review
- Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.Journal of bacteriology · 2005Article
- Active site restructuring regulates ligand recognition in class A penicillin-binding proteins.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- PBP1 is a component of the Bacillus subtilis cell division machinery.Journal of bacteriology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Previous studies have shown that Bacillus subtilis cells lacking penicillin-binding protein 1 (PBP1), encoded by ponA, have a reduced growth rate in a variety of growth media and are longer, thinner, and more bent than wild-type cells. It was also recently shown that cells lacking PBP1 require increased levels of divalent cations for growth and are either unable to grow or grow as filaments in media low in Mg2+, suggesting a possible involvement of PBP1 in septum formation under these conditions. Using epitope-tagging and immunofluorescence microscopy, we have now shown that PBP1 is localized at division sites in vegetative cells of B. subtilis. In addition, we have used fluorescence and electron microscopy to show that growing ponA mutant cells display a significant septation defect, and finally by immunofluorescence microscopy we have found that while FtsZ localizes normally in most ponA mutant cells, a significant proportion of ponA mutant cells display FtsZ rings with aberrant structure or improper localization, suggesting that lack of PBP1 affects FtsZ ring stability or assembly. These results provide strong evidence that PBP1 is localized to and has an important function in the division septum in B. subtilis. This is the first example of a high-molecular-weight class A PBP that is localized to the bacterial division septum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.