ArticleJournal of bacteriology1999
Enhanced function conferred on low-abundance chemoreceptor Trg by a methyltransferase-docking site.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 75 citations in OpenAlex.
- CheB localizes to polar receptor arrays during repellent adaptation.Science advances · 2024Article
- The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an Escherichia coli Cell.Journal of bacteriology · 2022Article
- Identification of Cyclic-di-GMP-Modulating Protein Residues by Bidirectionally Evolving a Social Behavior in Pseudomonas fluorescens.mSystems · 2022Article
- Evolutionary Divergence of the Wsp Signal Transduction Systems in Beta- and Gammaproteobacteria.Applied and environmental microbiology · 2021Article
- Article
- Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.Nature communications · 2021Article
- Paradoxical enhancement of chemoreceptor detection sensitivity by a sensory adaptation enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Variation of swimming speed enhances the chemotactic migration of Escherichia coli.Systems and synthetic biology · 2015Article
- Chemotactic Signaling by Single-Chain Chemoreceptors.PloS one · 2015Article
- Selective allosteric coupling in core chemotaxis signaling complexes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Salmonella chemoreceptors McpB and McpC mediate a repellent response to L-cystine: a potential mechanism to avoid oxidative conditions.Molecular microbiology · 2012Article
- Direct evidence that the carboxyl-terminal sequence of a bacterial chemoreceptor is an unstructured linker and enzyme tether.Protein science : a publication of the Protein Society · 2011Article
- Molecular modeling of flexible arm-mediated interactions between bacterial chemoreceptors and their modification enzyme.Protein science : a publication of the Protein Society · 2009Article
- Chemotaxis of Escherichia coli to pyrimidines: a new role for the signal transducer tap.Journal of bacteriology · 2008Article
- Functional analysis of nine putative chemoreceptor proteins in Sinorhizobium meliloti.Journal of bacteriology · 2007Article
- Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tethering.Molecular microbiology · 2007Article
- Uncovering a large set of genes that affect surface motility in Salmonella enterica serovar Typhimurium.Journal of bacteriology · 2006Article
- Diagnostic cross-linking of paired cysteine pairs demonstrates homologous structures for two chemoreceptor domains with low sequence identity.Protein science : a publication of the Protein Society · 2006Article
- Carboxyl-terminal extensions beyond the conserved pentapeptide reduce rates of chemoreceptor adaptational modification.Journal of bacteriology · 2005Article
- Ligand-specific activation of Escherichia coli chemoreceptor transmethylation.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Escherichia coli, high-abundance chemoreceptors are present in cellular amounts approximately 10-fold higher than those of low-abundance receptors. These two classes exhibit inherent differences in functional activity. As sole cellular chemoreceptors, high-abundance receptors are effective in methyl-accepting activity, in establishing a functional balance between the two directions of flagellar rotation, in timely adaptation, and in mediating efficient chemotaxis. Low-abundance receptors are not, even when their cellular content is increased. We found that the low-abundance receptor Trg acquired essential functional features of a high-abundance receptor by the addition of the final 19 residues of the high-abundance receptor Tsr. The carboxy terminus of this addition carried a methyltransferase-binding pentapeptide, NWETF, present in high-abundance receptors but absent in the low-abundance class. Provision of this docking site not only enhanced steady-state and adaptational methylation but also shifted the abnormal, counterclockwise bias of flagellar rotation toward a more normal rotational balance and vastly improved chemotaxis in spatial gradients. These improvements can be understood as the result of both enhanced kinase activation by the more methylated receptor and timely adaptation by more efficient methyl-accepting activity. We conclude that the crucial functional difference between the low-abundance receptor Trg and its high-abundance counterparts is the level of methyl-accepting activity conferred by the methyltransferase-docking site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.