ArticleJournal of bacteriology1999
Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Regulation of proteolysis of the sigma factor RpoS by the Gac-Rsm signal transduction system inMicrobiology (Reading, England) · 2026Article
- The Protein Quality Control Network inFrontiers in molecular biosciences · 2021Review
- Functional Characterization and Transcriptional Analysis of clpP of Xanthomonas campestris pv. campestris.Current microbiology · 2020Article
- The Protease ClpXP and the PAS Domain Protein DivL Regulate CtrA and Gene Transfer Agent Production in Rhodobacter capsulatus.Applied and environmental microbiology · 2018Article
- The Essential Role of ClpXP inFrontiers in molecular biosciences · 2017Article
- Finding approximate gene clusters with Gecko 3.Nucleic acids research · 2016Article
- Topological control of the Caulobacter cell cycle circuitry by a polarized single-domain PAS protein.Nature communications · 2015Article
- The global regulatory architecture of transcription during the Caulobacter cell cycle.PLoS genetics · 2015Article
- De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle.Genes & development · 2013Article
- Identification of ClpP substrates in Caulobacter crescentus reveals a role for regulated proteolysis in bacterial development.Molecular microbiology · 2013Article
- Temporal controls of the asymmetric cell division cycle in Caulobacter crescentus.PLoS computational biology · 2009Article
- Complex regulatory pathways coordinate cell-cycle progression and development in Caulobacter crescentus.Advances in microbial physiology · 2009Review
- High-throughput identification of transcription start sites, conserved promoter motifs and predicted regulons.Nature biotechnology · 2007Article
- Direct and adaptor-mediated substrate recognition by an essential AAA+ protease.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Expression of Arg-Gingipain RgpB is required for correct glycosylation and stability of monomeric Arg-gingipain RgpA from Porphyromonas gingivalis W50.Infection and immunity · 2005Article
- Identification of the protease and the turnover signal responsible for cell cycle-dependent degradation of the Caulobacter FliF motor protein.Journal of bacteriology · 2004Article
- Evidence for multiple levels of regulation of Oenococcus oeni clpP-clpL locus expression in response to stress.Journal of bacteriology · 2004Article
- Fine-tuning in regulation of Clp protein content in Bacillus subtilis.Journal of bacteriology · 2004Article
- The ATPase ClpX is conditionally involved in the morphological differentiation of Streptomyces lividans.Molecular genetics and genomics : MGG · 2003Article
- Lack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The region of the Caulobacter crescentus chromosome harboring the genes for the ClpXP protease was isolated and characterized. Comparison of the deduced amino acid sequences of the C. crescentus ClpP and ClpX proteins with those of their homologues from several gram-positive and gram-negative bacteria revealed stronger conservation for the ATPase regulatory subunit (ClpX) than for the peptidase subunit (ClpP). The C. crescentus clpX gene was shown by complementation analysis to be functional in Escherichia coli. However, clpX from E. coli was not able to substitute for the essential nature of the clpX gene in C. crescentus. The clpP and clpX genes are separated on the C. crescentus chromosome by an open reading frame pointing in the opposite direction from the clp genes, and transcription of clpP and clpX was found to be uncoupled. clpP is transcribed as a monocistronic unit with a promoter (PP1) located immediately upstream of the 5' end of the gene and a terminator structure following its 3' end. PP1 is under heat shock control and is induced upon entry of the cells into the stationary phase. At least three promoters for clpX (PX1, PX2, and PX3) were mapped in the clpP-clpX intergenic region. In contrast to PP1, the clpX promoters were found to be downregulated after heat shock but were also subject to growth phase control. In addition, the clpP and clpX promoters showed different activity patterns during the cell cycle. Together, these results demonstrate that the genes coding for the peptidase and the regulatory subunits of the ClpXP protease are under independent transcriptional control in C. crescentus. Determination of the numbers of ClpP and ClpX molecules per cell suggested that ClpX is the limiting component compared with ClpP.
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