ArticleOncogene1999
A mutant form of the rho protein can restore stress fibers and adhesion plaques in v-src transformed fibroblasts.
Article in Oncogene, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Activation Mechanism of RhoA Caused by Constitutively Activating Mutations G14V and Q63L.International journal of molecular sciences · 2022Article
- Small molecule modulation of the p75 neurotrophin receptor inhibits multiple amyloid beta-induced tau pathologies.Scientific reports · 2020Article
- Article
- Genomic landscape of cutaneous T cell lymphoma.Nature genetics · 2015Article
- Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas.Nature genetics · 2014Article
- Smooth muscle α-actin is a direct target of PLZF: effects on the cytoskeleton and on susceptibility to oncogenic transformation.Oncotarget · 2010Article
- ERK5 promotes Src-induced podosome formation by limiting Rho activation.The Journal of cell biology · 2008Article
- Disruption of c-Jun reduces cellular migration and invasion through inhibition of c-Src and hyperactivation of ROCK II kinase.Molecular biology of the cell · 2008Article
- Stable expression of intracellular Notch suppresses v-Src-induced transformation in avian neural cells.Oncogene · 2007Article
- Effects of constitutively active GTPases on fibroblast behavior.Cellular and molecular life sciences : CMLS · 2006Article
- Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function.The Journal of cell biology · 2004Article
- Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation.The Journal of cell biology · 2002Article
- p60(v-src) and serum control cell shape and apoptosis via distinct pathways in quail neuroretina cells.Oncogene · 2002Article
- Article
- Shp-2 mediates v-Src-induced morphological changes and activation of the anti-apoptotic protein kinase Akt.Oncogene · 2000Article
- Analysis of the signaling pathways regulating Src-dependent remodeling of the actin cytoskeleton.European journal of cell biologyArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The organization of polymerized actin in the mammalian cell is regulated by several members of the rho family. Three rho proteins, cdc42, rac and rho act in a cascade to organize the intracellular actin cytoskeleton. Rho proteins are involved in the formation of actin stress fibers and adhesion plaques in fibroblasts. During transformation of mammalian cells by oncogenes the cytoskeleton is rearranged and stress fibers and adhesion plaques are disintegrated. In this paper we investigate the function of the rho protein in RR1022 rat fibroblasts transformed by the Rous sarcoma virus. Two activated mutants of the rho protein, rho G14V and rho Q63L, and a dominant negative mutant, rho N1171, were stably transfected into RR1022 cells. The resulting cell lines were analysed for the organization of polymerized actin and adhesion plaques. Cells expressing rho Q63L, but not rho wt, rho G14V or rho N1171, showed an altered morphology. These cells displayed a flat, fibroblast like shape when compared with the RR1022 ancestor cells. Immunofluorescence analyses revealed that actin stress fibers and adhesion plaques were rearranged in these cells. We conclude from these data that an active rho protein can restore elements of the actin cytoskeleton in transformed cells by overriding the tyrosine kinase phosphorylation induced by the pp60(v-src).
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