ArticleProceedings of the National Academy of Sciences of the United States of America1999
The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 69 citations in OpenAlex.
- Arsenite and its trivalent methylated metabolites inhibit glucose-stimulated calcium influx and insulin secretion in murine pancreatic islets.Archives of toxicology · 2019Article
- Article
- Lessons from basic pancreatic beta cell research in type-2 diabetes and vascular complications.Diabetology international · 2017Article
- Islet adaptations in fetal sheep persist following chronic exposure to high norepinephrine.The Journal of endocrinology · 2017Article
- A Novel Diphenylthiosemicarbazide Is a Potential Insulin Secretagogue for Anti-Diabetic Agen.PloS one · 2016Article
- High heritability and genetic correlation of intravenous glucose- and tolbutamide-induced insulin secretion among non-diabetic family members of type 2 diabetic patients.Diabetologia · 2014Article
- High glucose and free fatty acids induce beta cell apoptosis via autocrine effects of ADP acting on the P2Y(13) receptor.Purinergic signalling · 2013Article
- Glucose-dependent docking and SNARE protein-mediated exocytosis in mouse pancreatic alpha-cell.Pflugers Archiv : European journal of physiology · 2011Article
- Dynamics of insulin secretion and the clinical implications for obesity and diabetes.The Journal of clinical investigation · 2011Review
- Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.Diabetologia · 2007Article
- Intracellular ATP-sensitive K+ channels in mouse pancreatic beta cells: against a role in organelle cation homeostasis.Diabetologia · 2006Article
- Fluid shear-induced ATP secretion mediates prostaglandin release in MC3T3-E1 osteoblasts.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2005Article
- The imidazoline NNC77-0020 affects glucose-dependent insulin, glucagon and somatostatin secretion in mouse pancreatic islets.Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells.The Journal of general physiology · 2003Article
- Water secretion associated with exocytosis in endocrine cells revealed by micro forcemetry and evanescent wave microscopy.Biophysical journal · 2002Article
- Expression and function of P-glycoprotein and absence of multidrug resistance-related protein in rat and beige mouse peritoneal mast cells.The Histochemical journal · 2001Article
- Tolbutamide stimulates exocytosis of glucagon by inhibition of a mitochondrial-like ATP-sensitive K+ (KATP) conductance in rat pancreatic A-cells.The Journal of physiology · 2000Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular application of the sulfonylurea tolbutamide during whole-cell patch-clamp recordings stimulated exocytosis >5-fold when applied at a cytoplasmic Ca2+ concentration of 0.17 microM. This effect was not detectable in the complete absence of cytoplasmic Ca2+ and when exocytosis was elicited by guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS). The stimulatory action could be antagonized by the sulfonamide diazoxide, by the Cl--channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), by intracellular application of the antibody JSB1 [originally raised against a 170-kDa multidrug resistance (mdr) protein], and by tamoxifen (an inhibitor of the mdr- and volume-regulated Cl- channels). Immunocytochemistry and Western blot analyses revealed that JSB1 recognizes a 65-kDa protein in the secretory granules. This protein exhibited no detectable binding of sulfonylureas and is distinct from the 140-kDa sulfonylurea high-affinity sulfonylurea receptors also present in the granules. We conclude that (i) tolbutamide stimulates Ca2+-dependent exocytosis secondary to its binding to a 140-kDa high-affinity sulfonylurea receptor in the secretory granules; and (ii) a granular 65-kDa mdr-like protein mediates the action. The processes thus initiated culminate in the activation of a granular Cl- conductance. We speculate that the activation of granular Cl- fluxes promotes exocytosis (possibly by providing the energy required for membrane fusion) by inducing water uptake and an increased intragranular hydrostatic pressure.
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