ArticlePlant physiology1999
Inhibition of nitrate uptake by ammonium in barley. Analysis Of component fluxes
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 152 citations in OpenAlex.
- Enhancing Crop Nitrogen Efficiency: The Role of Mixed Nitrate and Ammonium Supply in Plant Growth and Development.Biology · 2025Review
- The Dynamics of NO3- and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes.Plants (Basel, Switzerland) · 2021Article
- Co-Overexpression ofFrontiers in plant science · 2020Article
- Comparative study of the genetic basis of nitrogen use efficiency in wild and cultivated barley.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Hydroponic Solutions for Soilless Production Systems: Issues and Opportunities in a Smart Agriculture Perspective.Frontiers in plant science · 2019Review
- Time-Course of Metabolic and Proteomic Responses to Different Nitrate/Ammonium Availabilities in Roots and Leaves of Maize.International journal of molecular sciences · 2018Article
- Cross-Species Network Analysis Uncovers Conserved Nitrogen-Regulated Network Modules in Rice.Plant physiology · 2015Article
- Ecological significance and complexity of N-source preference in plants.Annals of botany · 2013Article
- Nitrogen stress affects the turnover and size of nitrogen pools supplying leaf growth in a grass.Plant physiology · 2013Article
- Net fluxes of ammonium and nitrate in association with H+ fluxes in fine roots of Populus popularis.Planta · 2013Article
- A pharmacological analysis of high-affinity sodium transport in barley (Hordeum vulgare L.): a 24Na+/42K+ study.Journal of experimental botany · 2012Article
- Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.).Journal of experimental botany · 2008Article
- Rapid, futile K+ cycling and pool-size dynamics define low-affinity potassium transport in barley.Plant physiology · 2006Article
- Ammonium transporter genes in Chlamydomonas: the nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression.Plant molecular biology · 2004Article
- Regulation of high-affinity nitrate transporter genes and high-affinity nitrate influx by nitrogen pools in roots of barley.Plant physiology · 2000Article
- Nitrate-ammonium synergism in rice. A subcellular flux analysisPlant physiology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NO3- uptake by plant roots is rapidly inhibited by exposure to NH4+. The rapidity of the effect has led to the presumption that the inhibition results from the direct effects of NH4+ at the plasma membrane. The mechanism of this inhibition, however, has been in contention. In the present study we used the radiotracer 13N to determine the relative effects of short-term exposures to NH4+ on the 13NO3- influx, efflux, and partitioning of absorbed 13N in barley (Hordeum vulgare) roots. Plants were grown without NO3- or NO2- (uninduced for NO3- uptake), or with 0.1, 1.0, 10 mM NO3-, or 0. 1 mM NO2- (to generate plant roots induced for NO3- uptake). Exposure to 1 mM NH4+ strongly reduced influx; the effect was most pronounced in plants induced for NO3- uptake when NO3- absorption was measured at low external NO3-. At higher [NO3-] and in uninduced plants the inhibitory effect was much diminished, indicating that NH4+ inhibition of influx was mediated via effects on the inducible high-affinity transport system rather than on the constitutive high-affinity transport system or the low-affinity transport system. Exposure to NH4+ also caused increased NO3- efflux; the largest effect was at low external [NO3-] in uninduced plants. In absolute terms, the reduction of influx made the dominant contribution to the observed reduction of net uptake of NO3-. Differences in response between plants induced with NO3- and those induced with NO2- indicate that NO2- may not be an appropriate analog for NO3- under all conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.