ArticlePlant physiology1999
Cloning and inactivation of genes encoding ferredoxin- and NADH-dependent glutamate synthases in the cyanobacterium plectonema boryanum. Imbalances In nitrogen and carbon assimilations caused by deficiency of the ferredoxin-dependent enzyme
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Optimisation of selection for methane mitigation by integrating production traits with ruminal microbiome-driven breeding in beef cattle.Genetics, selection, evolution : GSE · 2026Article
- An ancestral glutamate receptor mediates cell volume regulation during high KThe Journal of biological chemistry · 2026Article
- Nitrogen and phosphorus significantly alter growth, nitrogen fixation, anatoxin-a content, and the transcriptome of the bloom-forming cyanobacterium,Frontiers in microbiology · 2022Article
- Introduction of NADH-dependent nitrate assimilation in Synechococcus sp. PCC 7002 improves photosynthetic production of 2-methyl-1-butanol and isobutanol.Metabolic engineering · 2022Article
- Accessory Proteins of the Nitrogenase Assembly, NifW, NifX/NafY, and NifZ, Are Essential for Diazotrophic Growth in the Nonheterocystous CyanobacteriumFrontiers in microbiology · 2019Article
- Molecular insights into a dinoflagellate bloom.The ISME journal · 2017Article
- Crystallization and preliminary X-ray studies of an electron-transfer complex of ferredoxin and ferredoxin-dependent glutamate synthase from the cyanobacterium Leptolyngbya boryana.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Differential gene retention in plastids of common recent origin.Molecular biology and evolution · 2010Article
- Glutamate synthase: structural, mechanistic and regulatory properties, and role in the amino acid metabolism.Photosynthesis research · 2005Review
- Ammonium assimilation in cyanobacteria.Photosynthesis research · 2005Review
- Nitrogen control in cyanobacteria.Journal of bacteriology · 2001Review
- An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cyanobacterium.Photosynthesis research · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamate synthase (GOGAT) is a key enzyme in the assimilation of inorganic nitrogen in photosynthetic organisms. We found that, like higher plants, the facultative heterotrophic cyanobacterium Plectonema boryanum had ferredoxin (Fd)- and NADH-dependent GOGATs. The genes glsF, gltB, and gltD were cloned, and structural analyses and target mutageneses demonstrated that glsF encoded Fd-GOGAT and that gltB and gltD encoded the two subunits of NADH-GOGAT. All three mutants lacking one of the GOGAT genes were able to grow photosynthetically and heterotrophically. However, the Fd-GOGAT mutant exhibited a phenotype of marked nitrogen deficiency when grown under conditions of saturating illumination and CO2 supply. In these conditions the rate of the ammonia uptake from the culture medium was slower in the Fd-GOGAT mutant than in the wild type or in the NADH-GOGAT mutant, but no significant differences were found in the rate of the CO2 fixation-dependent O2 evolution among these strains. Our results suggest that, although both Fd- and NADH-GOGATs were operative in the cells growing in light, the contribution of Fd-GOGAT, which directly utilizes photoreducing power for the catalytic reaction, is essential for balancing photosynthetic nitrogen and carbon assimilation.
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