ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Different contributions of microtubule dynamics and transport to the growth of axons and collateral sprouts.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 114 citations in OpenAlex.
- NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- The response of Dual-leucine zipper kinase (DLK) to nocodazole: Evidence for a homeostatic cytoskeletal repair mechanism.PloS one · 2024Article
- Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics.Journal of cell science · 2023Article
- Microtubules are not required to generate a nascent axon in embryonic spinal neurons in vivo.EMBO reports · 2022Article
- The Role of Spastin in Axon Biology.Frontiers in cell and developmental biology · 2022Review
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- Cytoskeleton and Membrane Organization at Axon Branches.Frontiers in cell and developmental biology · 2021Review
- The bioenergetics of neuronal morphogenesis and regeneration: Frontiers beyond the mitochondrion.Developmental neurobiology · 2020Review
- Mechanism and role of the intra-axonal Calreticulin translation in response to axonal injury.Experimental neurology · 2020Article
- STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- The Role of the Microtubule Cytoskeleton in Neurodevelopmental Disorders.Frontiers in cellular neuroscience · 2018Review
- It takes a village to raise a branch: Cellular mechanisms of the initiation of axon collateral branches.Molecular and cellular neurosciences · 2017Review
- CSPGs inhibit axon branching by impairing mitochondria-dependent regulation of actin dynamics and axonal translation.Developmental neurobiology · 2017Article
- MAP7 Regulates Axon Collateral Branch Development in Dorsal Root Ganglion Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Beyond the cytoskeleton: The emerging role of organelles and membrane remodeling in the regulation of axon collateral branches.Developmental neurobiology · 2016Review
- Drebrin coordinates the actin and microtubule cytoskeleton during the initiation of axon collateral branches.Developmental neurobiology · 2016Article
- Actin filament-microtubule interactions in axon initiation and branching.Brain research bulletin · 2016Review
- Neuritin Mediates Activity-Dependent Axonal Branch Formation in Part via FGF Signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Axonal growth is believed to depend on microtubule transport and microtubule dynamic instability. We now report that the growth of axon collateral branches can occur independent of microtubule dynamic instability and can rely mostly on the transport of preassembled polymer. Raising embryonic sensory neurons in concentrations of either taxol or nocodazole (NOC) that largely inhibit microtubule dynamics significantly inhibited growth of main axonal shafts but had only minor effects on collateral branch growth. The collaterals of axons raised in taxol or nocodazole often contained single microtubules with both ends clearly visible within the collateral branch ("floating" microtubules), which we interpret as microtubules undergoing transport. Furthermore, in these collaterals there was a distoproximal gradient in microtubule mass, indicating the distal accumulation of transported polymer. Treatment of cultures with a high dose of nocodazole to deplete microtubules from collaterals, followed by treatment with 4-20 nM vinblastine to inhibit microtubule repolymerization, resulted in the time-dependent reappearance and subsequent distal accumulation of floating microtubules in collaterals, providing further evidence for microtubule transport into collateral branches. Our data show that, surprisingly, the contribution of microtubule dynamics to collateral branch growth is minor compared with the important role of microtubule dynamics in growth cone migration, and they indicate that the transport of microtubules may provide sufficient cytoskeletal material for the initial growth of collateral branches.
Indexed as
Identifiers
10234018PMC6782725W1543755059What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.