ArticleJournal of virology1999
Mutational analysis, using a full-length rubella virus cDNA clone, of rubella virus E1 transmembrane and cytoplasmic domains required for virus release.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Rubella virus mutations that confer resistance to inactivation at low pH.Journal of virology · 2025Article
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- Molecular and Structural Insights into the Life Cycle of Rubella Virus.Journal of virology · 2021Review
- Calcium-Dependent Rubella Virus Fusion Occurs in Early Endosomes.Journal of virology · 2016Article
- Rubella virus: first calcium-requiring viral fusion protein.PLoS pathogens · 2014Article
- Phosphorylation and membrane association of the Rubella virus capsid protein is important for its anti-apoptotic function.Cellular microbiology · 2014Article
- Identification of the myelin oligodendrocyte glycoprotein as a cellular receptor for rubella virus.Journal of virology · 2011Article
- The Rubella virus capsid is an anti-apoptotic protein that attenuates the pore-forming ability of Bax.PLoS pathogens · 2011Article
- Rubella virus capsid protein interacts with poly(a)-binding protein and inhibits translation.Journal of virology · 2008Article
- The cytoplasmic tails of Uukuniemi Virus (Bunyaviridae) G(N) and G(C) glycoproteins are important for intracellular targeting and the budding of virus-like particles.Journal of virology · 2007Article
- Analyses of phosphorylation events in the rubella virus capsid protein: role in early replication events.Journal of virology · 2006Article
- Analysis of rubella virus capsid protein-mediated enhancement of replicon replication and mutant rescue.Journal of virology · 2006Article
- Interactions between rubella virus capsid and host protein p32 are important for virus replication.Journal of virology · 2005Article
- Phosphorylation of rubella virus capsid regulates its RNA binding activity and virus replication.Journal of virology · 2003Article
- Rubella virus replication and links to teratogenicity.Clinical microbiology reviews · 2000Review
- Mutations in the E1 hydrophobic domain of rubella virus impair virus infectivity but not virus assembly.Journal of virology · 2000Article
- Mutational analysis of the rubella virus nonstructural polyprotein and its cleavage products in virus replication and RNA synthesis.Journal of virology · 2000Article
- Rubella virus nonstructural protein protease domains involved in trans- and cis-cleavage activities.Journal of virology · 2000Article
- A single-amino-acid substitution of a tyrosine residue in the rubella virus E1 cytoplasmic domain blocks virus release.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the construction of a full-length cDNA clone, pBRM33, derived from wild-type rubella virus M33 strain. The RNA transcripts synthesized in vitro from pBRM33 are highly infectious, and the viruses produced retain the phenotypic characteristics of the parental M33 virus in growth rate and plaque size. This cDNA clone was used to study the role of E1 transmembrane and cytoplasmic domains in virus assembly by site-directed mutagenesis. Three different alanine substitutions were introduced in the transmembrane domain of E1. These included substitution of leucine 464, cysteine 466, cysteine 467, and both cysteines 466 and 467 to alanine. In the E1 cytoplasmic domain, cysteine 470 and leucine 471 were altered to alanine. We found that these mutations did not significantly affect viral RNA replication, viral structural protein synthesis and transport, or E2/E1 heterodimer formation. Except for the substitution of cysteine 470, these mutations did, however, lead to a reduction in virus release. Substitution of cysteine 467 in the transmembrane region and of leucine 471 in the cytoplasmic domain dramatically reduced virus yield, resulting in the production of only 1 and 10% of the parental virus yield, respectively, in a parallel infection. These data show that E1 transmembrane and cytoplasmic domains play an important role in late stages of virus assembly, possibly during virus budding, consistent with earlier studies indicating that the E1 cytoplasmic domain may interact with nucleocapsids and that this interaction drives virus budding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.