ArticleImmunology1999
CD147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U937 via LFA-1/ICAM-1 pathway.
Article in Immunology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 87 citations in OpenAlex.
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- Mechano-induced patterned domain formation by monocytes.Nature materials · 2026Article
- Engineered CD147-CAR macrophages for enhanced phagocytosis of cancers.Cancer immunology, immunotherapy : CII · 2024Article
- The extracellular cyclophilin A-integrin β2 complex as a therapeutic target of viral pneumonia.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Sialylated Glycan Bindings from SARS-CoV-2 Spike Protein to Blood and Endothelial Cells Govern the Severe Morbidities of COVID-19.International journal of molecular sciences · 2023Review
- The retina-specific basigin isoform does not induce IL-6 expression in mouse monocytes.Molecular vision · 2023Article
- Immunoreactivity of humanized single-chain variable fragment against its functional epitope on domain 1 of CD147.Scientific reports · 2022Article
- A Deadly Embrace: Hemagglutination Mediated by SARS-CoV-2 Spike Protein at Its 22 N-Glycosylation Sites, Red Blood Cell Surface Sialoglycoproteins, and Antibody.International journal of molecular sciences · 2022Review
- Cell Aggregation Assays for Homophilic Interactions Between Cell Surface Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- New binding specificities evolve via point mutation in an invertebrate allorecognition gene.iScience · 2021Article
- Differential CD147 Functional Epitopes on Distinct Leukocyte Subsets.Frontiers in immunology · 2021Article
- Lactate and Lactate Transporters as Key Players in the Maintenance of the Warburg Effect.Advances in experimental medicine and biology · 2020Review
- CD147 mediates intrahepatic leukocyte aggregation and determines the extent of liver injury.PloS one · 2019Article
- Cluster of differentiation 147 mediates chemoresistance in breast cancer by affecting vacuolar HOncology letters · 2018Article
- The role of EMMPRIN in T cell biology and immunological diseases.Journal of leukocyte biology · 2015Review
- Function of CD147 in atherosclerosis and atherothrombosis.Journal of cardiovascular translational research · 2015Review
- Hepatocyte produced matrix metalloproteinases are regulated by CD147 in liver fibrogenesis.PloS one · 2014Article
- Review
- Production of monoclonal antibodies to P-glycoprotein: its application in detection of soluble and surface P-glycoprotein of leukemia patients.International journal of hematology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD147 is a 50 000-60 000 MW glycoprotein of the immunoglobulin superfamily broadly expressed on haemopoietic cell lines and peripheral blood cells. In the present study, six monoclonal antibodies (mAbs) directed against the CD147 protein were generated. The antigen defined by the generated CD147 mAbs is widely expressed on haemopoietic cell lines, peripheral blood cells and is a lymphocyte activation-associated cell surface molecule. The generated CD147 mAbs precipitated a broad protein band from U937 cells of 45 000-65 000 MW under reducing conditions. Functional analysis indicated that the CD147 mAbs markedly induced homotypic cell aggregation of U937 cells, but not K562 cells. The CD147 mAb-induced cell aggregation was inhibited by leucocyte function-antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) mAbs. However, the expression of LFA-1 and ICAM-1 molecules on U937 was not altered by CD147 mAb treatment. The U937 cell aggregation induced by CD147 mAb was also inhibited by ethylenediamine tetra-acetic acid (EDTA), sodium azide and when incubated at 4 degrees. We therefore propose that the binding of CD147 mAb to CD147 molecule, which mimics the natural ligand binding, may generate intracellular signals that activate LFA-1/ICAM-1 intercellular adhesion pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.