ArticleMolecular biology of the cell1999
In vivo observations of myosin II dynamics support a role in rear retraction.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 96 citations in OpenAlex.
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- Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium.Cell & bioscience · 2021Article
- Myosin-II-mediated directional migration of Dictyostelium cells in response to cyclic stretching of substratum.Biophysical journal · 2013Article
- Cell motility: the integrating role of the plasma membrane.European biophysics journal : EBJ · 2011Review
- The phosphorylation state of MRLC is polyamine dependent in intestinal epithelial cells.American journal of physiology. Cell physiology · 2011Article
- Expression of Y53A-actin in Dictyostelium disrupts the cytoskeleton and inhibits intracellular and intercellular chemotactic signaling.The Journal of biological chemistry · 2010Article
- How a cell crawls and the role of cortical myosin II.Eukaryotic cell · 2009Article
- Combining experiments and modelling to understand size regulation in Dictyostelium discoideum.Journal of the Royal Society, Interface · 2008Review
- Reversal of cell polarity and actin-myosin cytoskeleton reorganization under mechanical and chemical stimulation.Biophysical journal · 2008Article
- Myosin II co-chaperone general cell UNC-45 overexpression is associated with ovarian cancer, rapid proliferation, and motility.The American journal of pathology · 2007Article
- Rap1 controls cell adhesion and cell motility through the regulation of myosin II.The Journal of cell biology · 2007Article
- Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells.Molecular biology of the cell · 2007Article
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.Molecular biology of the cell · 2006Article
- Mathematically modelling the effects of counting factor in Dictyostelium discoideum.Mathematical medicine and biology : a journal of the IMA · 2006Article
- A Dictyostelium homologue of WASP is required for polarized F-actin assembly during chemotaxis.Molecular biology of the cell · 2005Article
- A cell number-counting factor regulates the cytoskeleton and cell motility in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The Dictyostelium LIM domain-containing protein LIM2 is essential for proper chemotaxis and morphogenesis.Molecular biology of the cell · 2000Article
- PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton in Dictyostelium discoideum cells during chemotaxis.The Journal of cell biology · 1999Article
- Neuronal migration illuminated: a look under the hood of the living neuron.Cell adhesion & migrationArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To investigate myosin II function in cell movement within a cell mass, we imaged green fluorescent protein-myosin heavy chain (GFP-MHC) cells moving within the tight mound of Dictyostelium discoideum. In the posterior cortex of cells undergoing rotational motion around the center of the mound, GFP-MHC cyclically formed a "C," which converted to a spot as the cell retracted its rear. Consistent with an important role for myosin in rotation, cells failed to rotate when they lacked the myosin II heavy chain (MHC-) or when they contained predominantly monomeric myosin II (3xAsp). In cells lacking the myosin II regulatory light chain (RLC-), rotation was impaired and eventually ceased. These rotational defects reflect a mechanical problem in the 3xAsp and RLC- cells, because these mutants exhibited proper rotational guidance cues. MHC- cells exhibited disorganized and erratic rotational guidance cues, suggesting a requirement for the MHC in organizing these signals. However, the MHC- cells also exhibited mechanical defects in rotation, because they still moved aberrantly when seeded into wild-type mounds with proper rotational guidance cues. The mechanical defects in rotation may be mediated by the C-to-spot, because RLC- cells exhibited a defective C-to-spot, including a slower C-to-spot transition, consistent with this mutant's slower rotational velocity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.