ArticleMolecular biology of the cell1999
Different domains of the M-band protein myomesin are involved in myosin binding and M-band targeting.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 88 citations in OpenAlex.
- Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics.Journal of biomolecular NMR · 2026Article
- Cardiomyopathy-associated and basic residue mutations in myopalladin alter actin binding, bundling, and structural stability.Protein science : a publication of the Protein Society · 2026Article
- Pathogenesis of Cardiomyopathy Caused by Variants inCirculation · 2022Article
- Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2.Cell reports · 2021Article
- The M-band: The underestimated part of the sarcomere.Biochimica et biophysica acta. Molecular cell research · 2020Review
- Thick Filament Protein Network, Functions, and Disease Association.Comprehensive Physiology · 2018Review
- The sarcomeric M-region: a molecular command center for diverse cellular processes.BioMed research international · 2015Review
- Structure and function of palladin's actin binding domain.Journal of molecular biology · 2013Article
- Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles.Journal of genetics and genomics = Yi chuan xue bao · 2012Article
- Structure and interactions of myosin-binding protein C domain C0: cardiac-specific regulation of myosin at its neck?The Journal of biological chemistry · 2011Article
- Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover.Developmental biology · 2011Article
- Three-dimensional structure of the M-region (bare zone) of vertebrate striated muscle myosin filaments by single-particle analysis.Journal of molecular biology · 2010Article
- Obscurin interacts with a novel isoform of MyBP-C slow at the periphery of the sarcomeric M-band and regulates thick filament assembly.Molecular biology of the cell · 2009Article
- A synthetic mammalian electro-genetic transcription circuit.Nucleic acids research · 2009Article
- Article
- Nuclear localization of Myomesin-1: possible functions.Journal of muscle research and cell motility · 2008Article
- M line-deficient titin causes cardiac lethality through impaired maturation of the sarcomere.The Journal of cell biology · 2006Article
- Cardiac muscle cell cytoskeletal protein 4.1: analysis of transcripts and subcellular location--relevance to membrane integrity, microstructure, and possible role in heart failure.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Stretchin-klp, a novel Drosophila indirect flight muscle protein, has both myosin dependent and independent isoforms.Journal of muscle research and cell motility · 2005Article
- The sarcomeric M-band during development and in disease.Journal of muscle research and cell motility · 2005Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myomesin is a 185-kDa protein located in the M-band of striated muscle where it interacts with myosin and titin, possibly connecting thick filaments with the third filament system. By using expression of epitope-tagged myomesin fragments in cultured cardiomyocytes and biochemical binding assays, we could demonstrate that the M-band targeting activity and the myosin-binding site are located in different domains of the molecule. An N-terminal immunoglobulin-like domain is sufficient for targeting to the M-band, but solid-phase overlay assays between individual N-terminal domains and the thick filament protein myosin revealed that the unique head domain contains the myosin-binding site. When expressed in cardiomyocytes, the head domains of rat and chicken myomesin showed species-specific differences in their incorporation pattern. The head domain of rat myomesin localized to a central area within the A-band, whereas the head domain of chicken myomesin was diffusely distributed in the cytoplasm. We therefore conclude that the head domain of myomesin binds to myosin but that this affinity is not sufficient for the restriction of the domain to the M-band in vivo. Instead, the neighboring immunoglobulin-like domain is essential for the precise incorporation of myomesin into the M-band, possibly because of interaction with a yet unknown protein of the sarcomere.
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