ArticleBiophysical journal1999
Electrorotation studies of baby hamster kidney fibroblasts infected with herpes simplex virus type 1.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Changes in peripheral blood mononuclear cell electrical properties in response to viral exposure and vaccination.Scientific reports · 2025Article
- A review of polystyrene bead manipulation by dielectrophoresis.RSC advances · 2019Review
- Membrane capacitance of thousands of single white blood cells.Journal of the Royal Society, Interface · 2017Article
- Multiple Roles of the Cytoplasmic Domain of Herpes Simplex Virus 1 Envelope Glycoprotein D in Infected Cells.Journal of virology · 2016Article
- Automated electrorotation shows electrokinetic separation of pancreatic cancer cells is robust to acquired chemotherapy resistance, serum starvation, and EMT.Biomicrofluidics · 2016Article
- Electrical response of a B lymphoma cell line latently infected with Kaposi's sarcoma herpesvirus.Biosensors & bioelectronics · 2016Article
- Article
- Microarray dot electrodes utilizing dielectrophoresis for cell characterization.Sensors (Basel, Switzerland) · 2013Article
- Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force.Biomicrofluidics · 2009Article
- Dielectrophoresis as a tool to characterize and differentiate isogenic mutants of Escherichia coli.Biophysical journal · 2006Article
- Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2004Article
- Assessment of multidrug resistance reversal using dielectrophoresis and flow cytometry.Biophysical journal · 2003Article
- Particle separation by dielectrophoresis.Electrophoresis · 2002Review
- Study of virus-cell interaction by the method of dielectrophoresis.Doklady. Biochemistry and biophysicsArticle
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Authors and funding
3 authors.
Funding
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Abstract
The dielectric properties of baby hamster kidney fibroblast (BHK(C-13)) cells have been measured using electrorotation before and after infection with herpes simplex virus type 1 (HSV-1). The dielectric properties and morphology of the cells were investigated as a function of time after infection. The mean specific capacitance of the uninfected cells was 2.0 microF/cm2, reducing to a value of 1. 5 microF/cm2 at 12 h after infection. This change was interpreted as arising from changes in the cell membrane morphology coupled with alterations in the composition of the cell membrane as infection progressed. The measured changes in the cell capacitance were correlated with alterations in cellular morphology determined from scanning electron microscope (SEM) images. Between 9 and 12 h after infection the internal permittivity of the cell exhibited a rapid change, reducing in value from 75epsilono to 58epsilono, which can be correlated with the generation of large numbers of Golgi-derived membrane vesicles and enveloped viral capsids. The data are discussed in relation to the known life cycle of HSV-1 and indicate that electrorotation can be used to observe dynamic changes in both the dielectric and morphological properties of virus-infected cells. Calculations of the dielectrophoretic spectrum of uninfected and infected cells have been performed, and the results show that cells in the two states could be separated using appropriate frequencies and electrode arrays.
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